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| Effects of Lactobacillus helveticus on Gene Expression in Intestinal Transcriptome of Loach Misgurnus anguillicaudatus |
| CUI Xiaolin1, DING Zhiwen1, MI Haoyu1, MENG Wenrong1, LIU Shu2, YANG Guang2, HOU Xiaoyue2, DONG Zhiguo1, FANG Yaowei2 |
1. College of Marine Science and Aquaculture, Jiangsu Ocean University, Lianyungang 222005, China;
2. College of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, China |
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Abstract In order to explore the effect of Lactobacillus helveticus HML037 on the growth and disease resistance gene expression of loach Misgurnus anguillicaudatus, the intestinal tissues of the loach from control group (C) and L. helveticus HML037 addition group (L) were double ended sequenced by Illumina hiseq 2500 high-throughput sequencing platform. The original data were quality controlled and de novo assembled, and 40 877 unigenes with an average length of 1218 bp and 1942 bp of N50. DEGseq was used to analyze gene expression difference, with 604 differential genes, of which 326 genes were significantly up-regulated and 278 genes were significantly down-regulated. All the differentially expressed genes were enriched in five pathways by GO functional enrichment analysis. The oxidation-reduction process pathway was the most abundant, followed by the oxidoreductase activity pathway, and the differentially expressed genes involved in these pathways were all up-regulated. Enrichment analysis of KEGG pathway showed that the differentially expressed genes were mainly concentrated in peroxidase proliferation-activation receptor signaling pathways, fatty acid prolongation and fat digestion and absorption. The findings provide an important reference for the improvement of intestinal growth and disease resistance performance of the loach by using L. helveticus HML037 instead of antibiotics.
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Received: 26 October 2020
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