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| Effects of Different Dissolved Oxygen Levels on Respiratory Metabolism and Related Gene Expression in Mandarinfish Siniperca chuatsi |
| SONG Yindu, ZENG Mengdong, ZHOU Haotian, ZHOU Yunhong, ZHANG Chengshuo, ZHAO Jinliang |
| Key Laboratory of Freshwater Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Collaborative Innovation Center, Aquatic Animal Genetics and Breeding Center, National Experimental Teaching Demonstration Center of Fisheries Science, Shanghai Ocean University, Shanghai 201306, China |
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Abstract In order to study the respiratory metabolism level of mandarinfish Siniperca chuatsi under different dissolved oxygen levels, the activities of pyruvate dehydrogenase (PDH), and lactate dehydrogenase (LDH) and the content of lactic acid (LA) in heart, brain, liver and muscle tissues, as well as the relative expression levels of related genes in each tissue were measured at different dissolved oxygen levels of 5 mg/L, 4 mg/L, 3 mg/L, 2 mg/L and 1 mg/L, and the oxygen consumption rate at each stage was calculated. The results showed that as the level of dissolved oxygen was decreased from 5 mg/L to 1 mg/L, the PDH activity and LDH activity in each tissue showed a downward and upward trend respectively, and the activities of PDH in liver and muscle were decreased significantly at 3 mg/L (P<0.05); while the activities of LDH in heart, muscle, brain, and liver were increased significantly at 2 mg/L (P<0.05); when the dissolved oxygen levels were reduced to 1 mg/L, the LA concentration in each tissues except liver was raised to the maximum level. In addition, expressions of LDH-A gene were significantly up-regulated in each tissues (P<0.001). Expression of PDH-E1α gene was significantly decreased in all tissues except heart (P>0.05). When the dissolved oxygen levels were reduced to 3 mg/L, the oxygen consumption rate was decreased very significantly (P<0.01). When the dissolved oxygen level was decreased, the mandarinfish reduced aerobic respiration, increased anaerobic respiration, and reduced the oxygen consumption rate to adapt to the hypoxic environment. When the dissolved oxygen level was less than 3 mg/L, the mandarinfish was under hypoxia.
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Received: 21 August 2020
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