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| Virulence Genes and Antibiotic Resistance Characteristics of Pathogenic Aeromonas veronii Isolated from Loach Misgurnus anguillicaudatus |
| HAN Yu1, PAN Jiwen1, WANG Xin1, YANG Nuo1, GUO Guiying2, LI Qian3, ZENG Jifeng4, ZHENG Jiping1 |
1.School of Life Sciences, Hainan University, Haikou 570228, China;
2.School of Science, Hainan University, Haikou 570228, China;
3.Network and Technology Center, Hainan University, Haikou 570228, China;
4.School of Animal Science and Technology, Hainan University, Haikou 570228, China |
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Abstract In order to understand the virulence gene carrier rate and drug resistance of Aeromonas veronii in Hainan and Guangdong provinces, the kidney, liver, and skin ulcers were collected from 46 samples of loach Misgurnus anguillicaudatus from 6 farms in the two regions. The samples were isolated from the bacteria, and 20 strains of A. veronii (7 from Hainan province and 13 from Guangdong province) were screened through morphological, physiological and biochemical identification and gyrA (gyrase A subunit)housekeeping gene sequence analysis, and their virulence genes and drug resistance genes and antibiotic susceptibility were tested. The results showed that the morphological, physiological and biochemical characteristics of the 20 isolated A. veronii were consistent with those reported in the "Handbook of Common Bacteria Identification"; the gyrA housekeeping gene sequence was consistent with that in the GenBank database. The spores had 90%－99% homology. Among the 20 strains of A. veronii, the detection rate of virulence genes such as aerolysin, enterotoxin, and protease was high in which the aerolysin gene and flagellin gene were detected as high as 100%, but the heat-stable enterotoxin gene was not detected; the detection rate of quinolone and sulfonamide drug resistance genes was 100%, while the amide alcohol, macrocyclic lipid and lincomycin drug resistance genes were not detected. The tolerance rate of erythromycin, norfloxacin, ampicillin and lincomycin and trimethoprim was 100%, highly sensitive to five drugs including aztreonam, gentamicin and cefotaxime. The findings provide a reference for the prevention and control of A. veronii in the production of loach M. anguillicaudatus.
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Received: 18 August 2020
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