DNA条形码在海蜇食物组成鉴定中的应用

doi:10.16378/j.cnki.1003-1111.20177

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Abstract Feeding analysis of edible jellyfish Rhopilema esculentum is helpful to understand its food sources and functional role. Comprehensively and accurately obtaining the information on food species is critical for this endeavor.However, information about the feeding types and food structures of jellyfish remains obscure because of the limitations of traditional methods. Unrestricted to the morphology of prey, DNA barcoding based method could get more accurate results efficiently. DNA Barcoding is not only used in initially proposed simple species identification, but also in studies of animal diets, food chain, and food web by using short DNA fragments extracted from feces and intestinal contents of animals.In this study, DNA barcoding method was used to analyze the diet of jellyfish in Liaodong Bay coastal waters to make more comprehensive analysis towards its diet compositions. COⅠ gene and ITS-5.8S rDNA were used as the target gene, and universal primers were designed to identify food composition of the jellyfish by DNA barcoding. A total of 36 effective COⅠ gene sequences were obtained and 4 species were identified, while 10 species from 30 effective ITS-5.8S rDNA sequences were identified by traditional sequencing. The results showed that using DNA barcoding identified some potentially food compositions species of the jellyfish, such as fish eggs/larvae, and planktonic larvae of shellfish. Howerer, those main preys of plankton were not found in traditional Sanger Sequencing on the dietary analysis of the jellyfish yet, which means the deficiency of Sanger Sequencing in analyzing food composition research. The finding provides reference for further study of jellyfish feeding.

Key words： Rhopilema esculentum DNA barcoding COⅠ ITS-5.8S rDNA

Received: 14 August 2020

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	Articles by authors
	LI Yulong
	BAO Xiangbo
	GAO Xianggang
	DUAN Yan
	WANG Bin
	DONG Jing
	LI Yunfeng

Cite this article:

LI Yulong,BAO Xiangbo,GAO Xianggang, et al. Application of DNA Barcoding in Analyzing Food Composition of Jellyfish Rhopilema esculentum[J]. Fisheries Science, 2022, 41(4): 614-621.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20177 OR https://www.shchkx.com/EN/Y2022/V41/I4/614

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