工厂化循环水养殖系统微生物群落结构和功能分析

doi:10.16378/j.cnki.1003-1111.20246

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Abstract In order to compare the differences in community composition, species diversity and function of bacteria in the recirculating aquaculture systems (RAS), bacterial species composition and predicted function in the water in fish production tanks, sedimentation filter, protein skimmer, biofilter tank and Sesuvium portulacastrum floating bed tank were analyzed by high-throughput sequencing technology. The results showed that compared with other tanks, the relative abundance of Alphaproteobacteria in biofilter and floating bed tanks were significantly reduced while Bacteroidetes markedly increased, with significantly higher Verrucomicrobia in biofilter tank. According to the genus level analysis, the proportion of dominant bacterium Loktanella in sedimentation filter and protein skimmer was 2—3 times as that in other tanks, meanwhile Rubritalea in biofilter tank was significantly higher than others, with the maximal species richness and diversity in floating bed tank, and the minimal flora abundance in the biofilter tank. There was the minimal similarity with other samples in terms of community composition and structure in the biofilter and floating bed tank. The PICRUSt functional prediction results showed that the dominant functional groups of bacterial communities in the RAS were mainly in the aspect of metabolism. Comparing the functional similarity of bacterial communities in each unit, it was found that the sedimentation tank and protein separation tank were similar, while the biofilter was similar to the floating bed tank. In addition, the relative abundance of nitrogen cycle function genes in floating bed tank was obviously higher. The findings can provide data and technical support for the construction of the RAS suitable for southern China.

Key words： recirculating aquaculture system bacterial community high-throughput sequencing

Received: 10 November 2020

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	Articles by authors
	XIONG Xiangying
	WANG Zhicheng
	LIU Xujia
	YAO Kunzhi
	LIANG Zhihui

Cite this article:

XIONG Xiangying,WANG Zhicheng,LIU Xujia, et al. Analysis of Microbial Community Structure and Function in Industrial Recirculating Aquaculture System[J]. Fisheries Science, 2022, 41(5): 810-819.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20246 OR https://www.shchkx.com/EN/Y2022/V41/I5/810

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