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| Virulence of Pathogenic Bacteria Causing Acute Hepatopancreas Necrosis Disease (AHPND) |
| LI Jiyun1,2, SHEN Hui2, MENG Qingguo1, WAN Xihe2, JIANG Ge2, QIAO Yi2, CHENG Jie2, FENG Yanqin2,3, LI Haolan1,2 |
1. College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210000, China;
2. Jiangsu Institute of Marine Fisheries, Nantong 226007, China;
3. College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang 222000, China |
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Abstract In recent years, acute hepatopancreas necrosis (AHPND) caused by pathogenic Vibrio parahaemolyticus (VPAHPND) has caused a high mortality rate of Pacific white leg shrimp Litopenaeus vannamei, which is divided into acute and subacute types from the course of the disease. In this study, six pathogenic V. parahaemolyticus strains were collected from different regions in Jiangsu province, which were used for verifying the virulence through challenge test and exploring the strain typing study through multilocus sequence typing (MLST). The results of the challenge test showed that the mortality of Pacific white leg shrimp in the challenge test group of strains SHY1669, SHY1776, SHY1777, and SHY1833 was significantly higher than that of strains SHY1673 and SHY1697 (P<0.05). The MLST showed that the types of strains SHY1669, SHY1673, SHY1697, SHY1776, SHY1777, and SHY1833 were ST452, ST882, ST415, ST114, ST919, and ST2355, among which ST2355 was a new sequence type, the rest were all I all known sequences, and had the highest homology with ST1743. Phylogenetic tree analysis showed that there was no significant differences in acute and subacute strains in evolution. Acute strains mainly appeared in Asia, while subacute strains appeared in North America and Asia. The findings indicate that different VPAHPND have different virulence (strong or weak), but they do not show differences in genetic relationship.
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Received: 01 December 2020
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