中草药及复方对嗜水气单胞菌体外抑菌作用研究

doi:10.16378/j.cnki.1003-1111.20069

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1013 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To effectively control the economic loss caused by infection of Aeromonas hydrophila from channel catfish Ictalurus punctatus, 41 kinds of extracts of Chinese herbal medicine were obtained by decoction, such as Flos Lonicerae, Spica Prunellae and Pericarpium Granati. The diameter of inhibition circle, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of 41 kinds of Chinese herbal medicine and their compound to A. hydrophila were detected respectively. The results showed that 14 of 41 Chinese herbs had bacteriostasis circle, with the minimal MIC in Rhizoma Coptidis at concentration of 7.81 mg/mL, comprehensive diameter of inhibition circle, MIC and MBC, comprehensive screening of 6 Chinese herbs with better bacteriostatic effect: Pericarpium Granati, Fructus Chebulae, Herba Violae, Lignum Sappan, Rhizoma Coptidis and Fructus Mume. Drug combination results showed that there was no antagonism of 93.3% between the 6 herbs, with positive effect of 80%. The results of 25 groups according to preparation tables of mixed Chinese herbal medicine showed that M-10 compound group with V (Pericarpium Granati)∶V (Fructus Chebulae)∶V (Herba Violae)∶V (Lignum Sappan)∶V (Rhizoma Coptidis)∶V (Fructus Mume) = 2∶5∶1∶2∶3∶4 had the best comprehensive bacteriostatic effect. The findings provide a theoretical basis for the application of Chinese herbal medicine in bacterial diseases of A. hydrophila.

Key words： Chinese herbal medicine Ictalurus punctatus Aeromonas hydrophila in vitro bacteriostatic effect

Received: 03 April 2020

S947.9

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	TAO Sha
	ZHANG Xiaoping
	SHANG Baodi
	LI Xiaoyi
	YAO Junjie

Cite this article:

TAO Sha,ZHANG Xiaoping,SHANG Baodi, et al. In Vitro Effect of Single and Compound Chinese Herbal Medicines on Bacteriostasis of Aeromonas hydrophila[J]. Fisheries Science, 2022, 41(5): 883-890.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20069 OR https://www.shchkx.com/EN/Y2022/V41/I5/883

[1]王洪彬,李永慧,朱利霞,等.24味中草药对舌鳎源嗜水气单胞菌的体外抑菌效果[J].水产学杂志,2018,31(1):21-24.
[2]周碧君,李永明,温贵兰,等.斑点叉尾鮰致病性嗜水气单胞菌的分离[J].水产科学,2004,23(12):9-12.
[3]卢静,王振宁,陈锐,等.几种中药单体和抗生素对嗜水气单胞菌及温和气单胞菌的体外抑菌活性研究[J].水生生物学报,2013,37(6):1128-1132.
[4]路晶晶,郭冉,齐国山,等.复方中草药对大菱鲆幼鱼生长性能及非特异性免疫指标的影响[J].大连海洋大学学报,2018,33(6):722-728.
[5]吕欣荣,肖克宇.中草药在水产养殖病害防治中的应用现状[J].内陆水产,2007,32(9):29-31.
[6]胡金城,刘金兰,吕爱军,等.中草药在水产养殖中的应用[J].天津水产,2016(1):15-22.
[7]刁菁,李乐,叶海斌,等. 一种中草药复方和11种单方的体外抑菌效果及对大菱鲆肠道菌群的影响[J].水产科学,2018,37(4):433-440.
[8]孟思妤,孟长明,陈昌福.水产养殖中控制抗生素类药物的使用(四)[J].科学养鱼,2015(9):87.
[9]丁瑞.中药抗病原微生物作用的现代研究进展[J].现代中西医结合杂志,2013,22(21):2387-2389.
[10]何欣,李诗言,王扬,等.基于高效液相色谱—四级杆—飞行时间质谱技术筛查渔业水域环境中抗生素残留方法的建立与应用[J].水产学报,2016,40(4):652-663.
[11]姜燕.凡纳滨对虾急性肝胰腺坏死病防治中草药的筛选[D].大连:大连海洋大学,2016:7-27.
[12]夏与晴,刘文珍,傅松哲,等.25种中草药及其联合用药配伍对5种水产养殖常见致病菌的抑菌作用[J].大连海洋大学学报,2019,34(1):7-14.
[13]杨移斌,曹海鹏,夏永涛,等.41种中草药对3种鲟源病原菌的体外抑菌效果[J].淡水渔业,2013,43(4):80-84.
[14]肖辉,苏振霞,单娟娟,等.16种中草药提取物对嗜水气单胞菌的体外抑菌实验[J].水生态学杂志,2009,30(3):53-56.
[15]孙俊颖,彭新宇,魏光伟.血清药理学在抗微生物中药研究上的应用[J].广东农业科学,2011,38(24):99-101.
[16]向双云,周珍辉,李玉冰,等.8种中草药对大肠杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2016(10):167-169.
[17]陈瑾,谭丽媛,张淑蓉,等.不同产地连翘主要成分分析及抗菌作用研究[J].时珍国医国药,2018,29(2):427-430.
[18]邓家刚,覃骊兰.试论中药配伍中的中医“和谐”观[J].时珍国医国药,2009,20(2):503-504.
[19]赵建荣,李晓玫.中药复方物质基础研究的现状与进展[J].中草药,2003,34(11):963-966.
[20]薛立喜.复方中草药对鹅源致病性大肠杆菌流行株体外抑菌活性研究[J].黑龙江畜牧兽医,2018(10):180-182.