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| Application of Environmental DNA in Monitoring of Fish Resources in Shaobo Lake |
| TANG Shengkai1, LIU Yanshan1, WANG Hua2, LI Daming1, ZHANG Tongqing1, SUN Jingying3, XU Fei2, WANG Zhihao3 |
1. Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China;
2. Gaobaoshaobohu Lake Fishery Management Commission of Jiangsu Province, Yangzhou 225009, China;
3. Nanjing E-Genomics Technology Co., Ltd., Nanjing 211100, China |
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Abstract Environmental DNA technology has been used in aquatic organisms monitoring in China, but rarely in lake fish monitoring. In order to explore the environmental DNA detection method suitable for fishes in shallow lakes, and to determine the composition of fish species in Shaobo Lake, water samples were collected synchronously at 15 sampling sites in November 2018, and fish species were detected by environmental DNA using mitochondrial DNA 12S rRNA and COⅠ primers. Compared with the two primers, the 12S rRNA primer was significantly better than the COⅠ primer for fish detection. The results showed that a total of 50 427 fish DNA sequences were detected by 12S rRNA primers, and that 71 fish operational taxonomic units (OTUs) were found by sequence alignment. However, only 990 fish DNA sequences were detected by COⅠ primers, and 16 fish OTUs were aligned. A total of 40 fish species (belonging to 6 orders and 12 families) were identified by eDNA metabarcoding, of which 36 species and 4 species were annotated as species and genus. The number of fish species detected at each site was ranged from 10 to 32, and common carp Cyprinus carpio, crucian carp Carassius auratus, Tanakia himantegus, bighead carp Aristichthys nobilis and Acheilognathus chankaensis had more sequences detected in these sites. Microphysogobio fukiensis and mullet Mugilogobius myxodermus were detected by environmental DNA in this study. Comparing with historical recorded based on morphological classification, the two species were first recorded in Shaobo Lake. The finding indicates that environmental DNA technology is characterized by high accuracy and sensitivity, without interference to the ecological environment. The technology is suitable for monitoring fish communities in shallow lakes.
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Received: 17 December 2020
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