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| Oxidative Damage of Microcystins to Hepatopancreas of Pacific White Shrimp Litopenaeus vannamei |
| HUANG Lanying, ZHANG Dajuan, ZHANG Shulin, BI Xiangdong, DAI Wei |
| Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China |
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Abstract Under the conditions of water temperature of (22±1) ℃, pH 8.0—8.5 and dissolved oxygen level of 7—8 mg/L, 20 μL of microcystins with the concentration of 3.52×10-4 mmol/kg was injected into Pacific white shrimp Litopenaeus vannamei with body weight of (16.0±1.0) g. The mortality of Pacific white shrimp was measured at 0, 2, 4, 8, 16, 24, 48 and 72 hours after injection, and hepatopancreas tissues were taken at the same time. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the changes in malondialdehyde (MDA) content were detected to study the effects of microcystins on the hepatopancreas of Pacific white shrimp. The results showed that the 72 h mortality rate was 13.33% in Pacific white shrimp after injection of microcystins. The content of malondialdehyde in hepatopancreas of Pacific white shrimp was increased gradually after injection of microcystins, which was significantly higher than that in the control group after 8 h, and reached the maximum value of (4.02±0.02) nmol/mg at 72 h (P<0.05). The activities of SOD, CAT and GSH-Px all showed a trend of increasing at first and then decreasing within 72 h, with the maximum at 2,4 and 8 h, respectively. Then the activity was gradually decreased, which was lower than that in the control group after 16 h, with the minimal value at 72 h. The findings indicated that microcystins gradually enhanced the lipid peroxidation in the hepatopancreas of Pacific white shrimp, inhibited the activities of antioxidant-related enzymes in Pacific white shrimp, and caused oxidative stress, causing severe oxidative damage to the hepatopancreas within 72 hours.
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Received: 26 January 2021
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