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| Sequence, Function and Expression of CCNC Gene in Ridgetail White Prawn Exopalaemon carinicauda |
| DAI Qin1, GAO Wei1, SHI Tingting1, CHEN Shuhan1, WANG Panpan1,2, LAI Xiaofang1,2, GAO Huan1,2,3, YAN Binlun1,2 |
1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China;
2. The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China;
3. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China |
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Abstract Ridgetail white prawn Exopalaemon carinicauda is an economically important shrimp species along the coast of China. Cyclin C (CCNC) as a member of the cyclin family plays an important role in cell cycle regulation and transcription regulation. In order to study the biological function of CCNC in ovarian and larval development of ridgetail white prawn, the cDNA sequence of EcCCNC gene was obtained using rapid amplification of cDNA ends method (RACE). The relative expression levels of EcCCNC gene were analyzed in different tissues and at different stages of ovarian and larval development by qRT-PCR. Recombinant protein was obtained by prokaryotic expression. The results showed that the full-length cDNA sequence of EcCCNC was 1042 bp, which contained a 5′-UTR of 82 bp, a 3′-UTR of 156 bp, and a 804 bp open reading frame (ORF) encoding 267 amino acids. The predicted molecular weight of the protein was 31.34kDa, and the theoretical isoelectric point was 5.50. Cluster analysis showed that EcCCNC and CCNC in Pacific white shrimp Litopenaeus vannamei were clustered. The amino acid sequence also had the highest homology with Pacific white shrimp (92%). EcCCNC had the highest relative expression levels in the ovary, especially the ovarian stage Ⅲ and the larval stage Ⅱ (P<0.05). The recombinant protein of EcCCNC was 45 ku and had a His tag. The findings indicate that EcCCNC may play an important role in the development of ovary and larva of ridgetail white prawn, which provides a theoretical basis for further understanding of the mechanism of shrimp ovarian and larval development.
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Received: 02 February 2021
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