|
|
|
| Comparative Analysis on Intestinal Microflora of Wild and Cultured Tench Tinca tinca |
| QIAN Yi1, XU Qinyu1, QIAN Long2, AI Tao2, XIANG Wei2, HU Bolin2, WANG Jun1, WANG Chenghui1 |
1. Shanghai Engineering Research Center of Aquaculture, National Demonstration Centre for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources Certificated by Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
2. Fisheries Technology Extension Station, Xinjiang Production and Construction Corps, Ürümqi 830002, China |
|
|
|
|
Abstract The bacterial community and function of the intestinal microbiota play important roles in the growth and development of fish. Tench Tinca tinca is a cultured fish species with high economic value in Xinjiang area, China. In order to compare the structure and abundance of intestinal microbial communities between the wild and cultured tench, the 16S rRNA gene (V3—V4 region) was sequenced between wild and cultured tench using Illumina Miseq high-throughput sequencing method. The results showed that the dominant intestinal microbial flora in tench was mainly Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota. However, significant intestinal microbiota was identified between wild and cultured tench. In detail, at phylum level, the abundance of Fusobacteriota and Acidobacteriota in the cultured tench was significantly lower than that in the wild tench (P<0.05). At genus level, the abundance of Lactobacillus, and Acinetobacter was higher in the cultured tench than that in the wild tench, and the abundance of Mycoplasmataceae and Flavobacterium were lower in the cultured tench (P＜0.01). At species level, the abundance of A. lwoffii in the cultured tench was higher than that in the wild tench (P＜0.01), while the unclassified Flavobacterium was significantly lower in the wild tench (P＜0.05). It was found that the community and abundance of the intestinal microbiome was significantly different between wild and cultured tench. Our findings provide basic data for the research of intestinal microbiome and cultivation management of tench in future.
|
|
Received: 19 April 2021
|
|
|
|
|
|
[1]冯晓宇,李忠全,李行先,等.丁鱥(Tinca tinca L.)引种驯养和人工繁育的初步研究[J].水产科技情报,2002,29(6):248-251.
[2]范镇明,钱龙向,伟艾涛,等.丁鱥仔幼鱼的形态特征观察[J].淡水渔业,1999,29(8):25-26.
[3]任慕莲,郭焱, 张人铭, 等.中国额尔齐斯河鱼类资源及渔业[M].乌鲁木齐:新疆科技卫生出版社,2002.
[4]于潇,王延晖,贺海战,等.丁鱥鱼与黄河鲤杂交种肌肉营养成分分析[J].中国水产,2020(8):79-81.
[5]祖岫杰,刘艳辉,李改娟,等.欧洲丁鱥规模化人工繁殖技术研究[J].吉林水利,2012(4):42-45.
[6]林旭元,李爱民,艾涛,等.丁鱥苗种循环水保种培育试验[J].新疆农垦科技,2020,43(9):25-27.
[7]ZENG M Y, CISALPINO D, VARADARAJAN S, et al. Gut microbiota-induced immunoglobulin G controls systemic infection by symbiotic bacteria and pathogens[J].Immunity,2016,44(3):647-658.
[8]TRUST T J, SPARROW R A. The bacterial flora in the alimentary tract of freshwater salmonid fishes[J].Canadian Journal of Microbiology,1974,20(9):1219-1228.
[9]徐晟云.不同环境下鳢肠道菌群特征及饲料中添加乳酸菌的效果研究[D].上海:上海海洋大学,2020.
[10]郁二蒙,张振男,夏耘,等.摄食不同饵料的大口黑鲈肠道菌群分析[J].水产学报,2015,39(1):118-126.
[11]刘增新.牙鲆(Paralichthys olivaceus)仔稚幼鱼肠道菌群结构变化及定植规律研究[D].上海:上海海洋大学,2017.
[12]陈孝煊,吴志新,周文豪.鱼类消化道菌群的作用与影响因素研究进展[J].华中农业大学学报,2005,24(5):523-528.
[13]李秀玲,刘宝锁,刘波,等.不同脂肪源对卵形鲳鲹肠道微生物菌群的影响[J].水产科学,2020,39(1):88-95.
[14]PULA H J, TRENZADO C E, GARCÍA-MESA S, et al. Effects of different culture systems on growth, immune status, and other physiological parameters of tench (Tinca tinca)[J].Aquaculture,2018,485:101-110.
[15]李可俊,管卫兵,徐晋麟,等.PCR-DGGE对长江河口八种野生鱼类肠道菌群多样性的比较研究[J].中国微生态学杂志,2007,19(3):268-269.
[16]XIA J H, LIN G, FU G H, et al. The intestinal microbiome of fish under starvation[J].BMC Genomics,2014,15:266.
[17]王瑞旋,王江勇,徐力文,等.军曹鱼肠道及水体异养菌和弧菌的周年变化[J].中国水产科学,2008,15(6):1008-1015.
[18]陈鹏,孙启睿,张宏华,等.基于16S rRNA基因分析异育银鲫肠道微生物多样性[J].水产科学,2022,41(2):266-272.
[19]KIM D H, BRUNT J, AUSTIN B. Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)[J].Journal of Applied Microbiology,2007,102(6):1654-1664.
[20]FJELLHEIM A J, PLAYFOOT K J, SKJERMO J, et al. Vibrionaceae dominates the microflora antagonistic towards Listonella anguillarum in the intestine of cultured Atlantic cod (Gadus morhua L.) larvae[J].Aquaculture,2007,269(1/2/3/4):98-106.
[21]翟万营,郭安宁.鱼类肠道微生物研究进展[J].河南水产,2016(4):18-21.
[22]朱钦士.肥胖与肠道细菌有关吗[J].生物学通报,2013,48(12):20-25.
[23]何旭云,贺姣姣,郑宁宁,等.黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究[J].世界中医药,2016,11(11):2379-2384.
[24]MICHAUD L, LO GIUDICE A, TROUSSELLIER M, et al. Phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system[J].Journal of Applied Microbiology,2009,107(6):1935-1946.
[25]吴金芳,张雨潇,彭仁.红球菌的组学、遗传改造及其应用研究进展[J].应用与环境生物学报,2022,28(1):239-246.
[26]皮静,严培文,杜博兴,等.青枯雷尔氏菌运动性相关研究进展[J].植物医生,2020,33(5):18-21.
[27]WANG L, ZHOU H H, HE R J, et al. Effects of soybean meal fermentation by Lactobacillus plantarum P8 on growth, immune responses, and intestinal morphology in juvenile turbot (Scophthalmus maximus L.)[J].Aquaculture,2016,464:87-94.
[28]GIRI S S, SUKUMARAN V, OVIYA M. Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita[J].Fish & Shellfish Immunology,2013,34(2):660-666.
[29]LIU Y, FENG L, JIANG J, et al. Effects of dietary protein levels on the growth performance, digestive capacity and amino acid metabolism of juvenile Jian carp (Cyprinus carpio var. jian)[J].Aquaculture Research,2009,40(9):1073-1082.
[30]LEE H, GILLILAND S, CARTER S. Amylolytic cultures of Lactobacillus acidophilus:potential probiotics to improve dietary starch utilization[J].Journal of Food Science,2001,66(2):338-344.
[31]ZHAO X, GÄNZLE M G. Genetic and phenotypic analysis of carbohydrate metabolism and transport in Lactobacillus reuteri[J].International Journal of Food Microbiology,2018,272:12-21.
[32]WATSON D, O'CONNELL MOTHERWAY M, SCH-OTERMAN M H C, et al. Selective carbohydrate utilization by lactobacilli and bifidobacteria[J].Journal of Applied Microbiology,2013,114(4):1132-1146.
[33]CALVEZ S, NAVARRO-GONZALEZ N, SIEKOULA-NGUEDIA C, et al. High genetic diversity in Flavobacterium psychrophilum isolates from healthy rainbow trout (Oncorhynchus mykiss) farmed in the same watershed, revealed by two typing methods[J].Applied and Environmental Microbiology,2021,87(2):e01398-e01320.
[34]孙坤,王晓磊,张洁,等.基于16S rRNA高通量测序评价患病草鱼体表及肝脏细菌多样性[J].水产科学,2022,41(2):202-209.
[35]邓时铭,刘丽,褚武英,等.中华鳖疥疮病原菌的分离鉴定及病理组织观察[J].福建农林大学学报(自然科学版),2020,49(5):660-665.
[36]顾泽茂,柳阳,陈昌福,等.鲍曼不动杆菌斑点叉尾鮰株的分离与鉴定[J].华中农业大学学报,2010,29(4):489-493.
[37]毛芝娟,毛甬州,汪建萍.两株鱼源琼氏不动杆菌的分离、鉴定和耐药特性分析[J].水产学报,2013,37(10):1572-1578.
[38]樊利虹,郭红瑞,吴江,等.牛源皮特不动杆菌对小鼠的致病性分析[J].浙江农业学报,2021,33(2):230-238.
[39]吴春艳,任思宇,汪开毓,等.一株虹鳟源乌尔新不动杆菌的分离鉴定与其感染的病理损伤[J].水产科学,2019,38(6):797-803.
[40]林杰,王旭荣,王磊,等.奶牛生乳中洛菲不动杆菌的分离鉴定与耐药性分析[J].西北农业学报,2016,25(6):811-815. |
|
|
|
