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| Effects of Temperature on Oxygen Consumption Rate, Suffocation Threshold, and Antioxidant Capacity of Juvenile Chinese Hooksnout Carp Opsariichthys bidens |
| ZHANG Kai1,2, WANG Weiyu1, ZHAO Liyue1, XIONG Luomei1, MEI Yehan1, HUANG Kexin1, LIU Fangling1,2, YU Jin3, ZHENG Shanjian1,2 |
1. College of Chemistry and Bioscience, Zhejiang Normal University, Jinhua 321004, China;
2. Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China;
3. Longyou County Aquaculture Development Center, Quzhou 324000, China |
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Abstract In order to explore the oxygen consumption rate, suffocation threshold, and antioxidant index of two sizes of juveniles Chinese hooksnout carp Opsariichthys bidens in different cultural environments, the oxygen consumption rate, suffocation point, and anti-oxidation indices of Chinese hooksnout carp were determined under three temperature gradients of 20, 24 and 28 ℃. It was found that the oxygen consumption rate and suffocation point of the juveniles was increased first and then decreased with increasing temperature. The oxygen consumption rate and suffocation point of small-sized juveniles (1.22±0.31) g at 20 ℃ were (0.185±0.057) mg/(g·h) and (1.149±0.110) mg/L, significantly lower than the consumption at 24 ℃ and 28 ℃(P<0.05). The suffocation threshold of small-sized juveniles was significantly higher at 24 ℃ than that at 28 ℃(P<0.05), but there was no significant difference in oxygen consumption rate(P>0.05); the oxygen consumption rate of large-sized juveniles(4.91±0.55) g under three temperature gradients was 24 ℃>28 ℃>20 ℃, and the suffocation point was 28 ℃>24 ℃>20 ℃. Under the condition of 28 ℃, there was no significant difference in the oxygen consumption rate of two kinds of juvenile fish(P>0.05). Under hypoxia stress, the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and total antioxidant capacity (T-AOC) in liver tissues of the juvenile were significantly increased (P<0.05), and the SOD activity and T-AOC content of the liver of the juvenile were higher than those of the small ones (P<0.05). The findings indicate that the adaptability of small-sized juveniles to the environment is lower than that of large-sized juveniles, and they are more susceptible to damage under hypoxia and temperature stress.
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Received: 11 May 2021
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