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| Effects of Curcumin on Growth Performance and Non-Specific Immunity of Largemouth Bass Micropterus salmoides |
| ZHANG Meiyan1,2, YANG Xing1,2, JIANG Zaisheng3, ZHAO Feng1,2, LI Songyan4, LIU Tingting4, ZHANG Zhixin4, SHANG Baodi1,2 |
1. Institute of Fisheries, Guizhou Academy of Agricultural Sciences, Guiyang 550025; China;
2. Special Aquaculture of Engineering Technology Center of Guizhou, Guiyang 550025, China;
3. Extending Stations of Aquaculture Technology of Agriculture and Rural Bureau in Xuanzhou in Anhui, Xuancheng 242000, China;
4. Guizhou Normal University, Guiyang 550018, China |
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Abstract The 60-day feeding trial was conducted to study the effects of different dietary curcumin levels on growth performance, and non-specific immunity for largemouth bass Micropterus salmoides with body weight of (4.17±0.39) g. Purified basal diets were formulated, containing curcumin of 0(control), 15, 30, 60, 120, and 240 mg/kg diet. Each diet was fed to triplicate groups, 30 fish each group. The results showed that: (1)compared with the control group, the weight gain rate (WGR) and specific growth rate (SGR) were significantly higherin 60 mg/kg groupthan that in the others (P<0.05), and the FCR(feed conversion ratio) was lowest in 30 mg/kg and 60 mg/kg groups, but there was no significant differences in viscerosomatic index (VSI), hepatosomatic index (HSI) among the experimental groups (P>0.05). (2)White blood cell (WBC) number were significantly higherin 30 mg/kg and 60 mg/kg groupsthan that in control group (P<0.05), there was no significant differences in red blood cell (RBC) and HGB (hemoglobin) among all groups (P>0.05). (3)With the increase in dietary curcumin supplementation, the alanine amino transferase (ALT) and aspartate amino transferase (AST) activities were decreased first and then increased, alanine amino transferase (ALT) reached the lowest level when dietary curcumin supplementation was 60 mg/kg than others (P<0.05), and aspartate amino transferase (AST) reached the lowest level when dietary curcumin supplementation were 60 mg/kg and 120 mg/kg(P<0.05). There were no significant differences in total protein (TP) and globular proteins (GLB) among groups (P>0.05). (4)Compared with the control group, the activities of ACH50 in 30, 60 and 120 mg/kg groups were significantly higher (P<0.05), and in groups with curcumin TNF-α were significantly higher than that in the control group (P<0.05). The IL-1β activity was the highest in 60 mg/kg group. (5)In 60 mg/kg group, the expression of PrxⅠ, PrxⅡ, PrxⅣ, PrxⅤ, and PrxⅥ genes was significantly increased (P<0.05). In conclusion, the dietary with 60 mg/kg curcumin can improve the growth performance, non-specific immune response and antioxidant capacity of M. salmoides, which is the optimal dietary supplemental level.
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Received: 21 July 2021
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