青海湖裸鲤FXYD基因家族鉴定、分子进化及表达

doi:10.16378/j.cnki.1003-1111.21243

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Abstract Tibetan naked carp Gymnocypris przewalskii ssp. przewalskii is the only species of Schizothoracinae (Cyprinidae), which could adapt to the high saline-alkaline water environment in the Qinghai Lake. In the present study, we identified 16 FXYD genes in G. przewalskii ssp. przewalskii based on the G. przewalskii ssp. przewalskii genome. FXYD (Phen-XAA-Tyr-ASP) gene family encodes a group of small transmembrane proteins that regulate ion channel activities in responses to saline stress in a variety of organisms. Multiple sequence alignment and phylogenetic analysis revealed that 16 gpFXYD genes were divided into five subfamilies. Collinearity analysis indicated that 12 family members of the gpFXYD gene family were originated from genome-wide duplication, which showed synteny with their counterparts in zebrafish. This result suggested that the gain of extra copies of FXYD genes might be attributed to the whole genome duplication in G. przewalskii ssp. przewalskii. Molecular evolution analysis showed that the K_a/K_s values of gpFXYD1b/1c and gpFXYD5c/5d were greater than 1, which indicates that the positive selection might occur during evolution, leading to adaptive evolution. The subfamilies of gpFXYDs were expressed in diverse tissues, including heart, hepatopancreas, brain, kidney, gill, intestine and gonad. gpFXYD1s and gpFXYD7s were specifically expressed in the heart and brain. The results indicated that the function of gpFXYD subfamilys was in a tissue-dependent manner. After the transition from freshwater to saline water condition, the expression of gpFXYD5s, gpFXYD6s and gpFXYD11s showed significant changes in kidney and gill. The transcriptional variations might contribute to the ion transport and osmoregulation in G. przewalskii ssp. przewalskii at high salinity. The current study laid a foundation for understanding the adaptation of G. przewalskii ssp. przewalskii to high salinity environment, providing new insights into the molecular breeding of aquatic animals.

Key words： Gymnocypris przewalskii ssp. przewalskii FXYD gene family transcriptional profile salinity adaptation

Received: 24 November 2021

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	Articles by authors
	ZHOU Bingzheng
	CHEN Shengxue
	LIU Sijia
	QI Delin
	QI Hongfang
	WANG Yang
	ZHAO Kai
	TIAN Fei

Cite this article:

ZHOU Bingzheng,CHEN Shengxue,LIU Sijia, et al. Genome-Wide Identification, Molecular Evolution, and Expression of FXYD Gene Family in Tibetan Naked Carp Gymnocypris przewalskii ssp. przewalskii[J]. Fisheries Science, 2023, 42(5): 760-774.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21243 OR https://www.shchkx.com/EN/Y2023/V42/I5/760

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