|
|
|
| Cloning and mRNA Expression Analysis of C1q Gene in Sea Cucumber Apostichopus japonicus |
| CHEN Zhong |
| Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China |
|
|
|
|
Abstract C1q, as the complement recognition protein of the classical activation pathway, is very important for the immunity of the body. In order to study the role of C1q gene in the immune defense of sea cucumber Apostichopus japonicus, the full-length cDNA sequence of sea cucumber C1q (AjC1q) cDNA was cloned by the homologous gene cloning and RACE, and other molecular biology techniques. The full-length cDNA sequence was 1717 bp, in which the 5'- terminal untranslated region was 110 bp, the 3-terminal untranslated region was 698 bp, and the open reading frame was 909 bp, encoding 302 amino acids. AjC1q protein has a globular C1q domain and is a trimer, which has high homology with the C1q domain of most animals in wild. The mRNA of AjC1q was expressed in the whole development of sea cucumber. The expression increased significantly in the doliolaria stage and reached the peak in the pentactula stage, indicating that the complement system may be widely activated in the doliolaria stage. And the mRNA of AjC1q was expressed in different tissues and was highly expressed in tube feet. In addition, the AjC1q gene could be up-regulated in a short time after stimulation by different pathogens, indicating that AjC1q is rapidly expressed under the stimulation of pathogens. So AjC1q may play an important role in the development of sea cucumber and in the immune response.
|
|
Received: 06 July 2022
|
|
|
|
|
|
[1] 廖玉麟.中国动物志:棘皮动物门:海参纲[M].北京:科学出版社,1997:148-150.
[2] LOVATELLI, A., CONAND, C, PURCELL, S, et al. Advances in sea cucumber aquaculture and management. FAO Fisheries Technical Paper 463[M].Rome:FAO,2004:1-425.
[3] HAN Q X, KEESING J K, LIU D Y. A review of sea cucumber aquaculture, ranching, and stock enhancement in China[J].Reviews in Fisheries Science & Aquaculture,2016,24(4):326-341.
[4] ZHANG W W, LI C H, GUO M. Use of ecofriendly alternatives for the control of bacterial infection in aquaculture of sea cucumber Apostichopus japonicus[J].Aquaculture,2021,545:737185.
[5] 柯亚夫.海参的研究[M].青岛:中国海洋大学出版社,2010:108-111.
[6] CABELLO F C. Heavy use of prophylactic antibiotics in aquaculture:a growing problem for human and animal health and for the environment[J].Environmental Microbiology,2006,8(7):1137-1144.
[7] CAMPBELL D A, PANTAZIS P, KELLY M S. Impact and residence time of oxytetracycline in the sea urchin, Psammechinus miliaris, a potential aquaculture species[J].Aquaculture,2001,202(1/2):73-87.
[8] KMMERER K. Antibiotics in the aquatic environ-ment-A review-Part Ⅱ[J].Chemosphere,2009,75(4):435-441.
[9] 王易主.免疫学导论[M].上海:上海中医药大学出版社,2007:36-53.
[10] 谷珉.仿刺参(Apostichopus japonicus)免疫增强剂的体外筛选与养殖试验的评估[D].青岛:中国海洋大学,2010:1-2.
[11] MEDZHITOV R, JANEWAY JR C A. Innate immune recognition and control of adaptive immune responses[J].Seminars in Immunology,1998,10(5):351-353.
[12] CARROLL M C. The complement system in regulation of adaptive immunity[J].Nature Immunology,2004,5(10):981-986.
[13] CARROLL M V, SIM R B. Complement in health and disease[J].Advanced Drug Delivery Reviews,2011,63(12):965-975.
[14] HAJISHENGALLIS G, REIS E S, MASTELLOS D C, et al. Novel mechanisms and functions of complement[J].Nature Immunology,2017,18(12):1288-1298.
[15] RICKLIN D, HAJISHENGALLIS G, YANG K, et al. Complement:a key system for immune surveillance and homeostasis[J].Nature Immunology,2010,11(9):785-797.
[16] ARBORE G, KEMPER C, KOLEV M. Intracellular complement the complosome in immune cell regulation[J].Molecular Immunology,2017,89:2-9.
[17] RICKLIN D, REIS E S, LAMBRIS J D. Complement in disease:a defence system turning offensive[J].Nature Reviews Nephrology,2016,12(7):383-401.
[18] VENKATRAMAN GIRIJA U, GINGRAS A R, MARSHALL J E, et al. Structural basis of the C1q/C1s interaction and its central role in assembly of the C1 complex of complement activation[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(34):13916-13920.
[19] LU J H, TEH B K, WANG L D, et al. The classical and regulatory functions of C1q in immunity and autoimmunity[J].Cellular & Molecular Immunology,2008,5(1):9-21.
[20] KISHORE U, GABORIAUD C, WATERS P, et al. C1q and tumor necrosis factor superfamily:modularity and versatility[J].Trends in Immunology,2004,25(10):551-561.
[21] KISHORE U, REID K B M. Modular organization of proteins containing C1q-like globular domain[J].Immunopharmacology,1999,42(1/2/3):15-21.
[22] KISHORE U, GHAI R, GREENHOUGH T J, et al. Structural and functional anatomy of the globular domain of complement protein C1q[J].Immunology Letters,2004,95(2):113-128.
[23] 张宁.文昌鱼和斑马鱼C1q、C3及G10的进化分析及基因表达的研究[D].济南:山东大学,2009:50.
[24] 孙红娟,陈仲,贺迪,等.仿刺参SARM基因的克隆、表达和功能研究[J].水产科学,2021,40(1):1-9.
[25] REN L Y, LI K Q, ZHAN Y Y, et al. Isolation of a new PAK1 gene from sea cucumber (Apostichopus japonicus) and its expression analysis and function characterization[J].Journal of Ocean University of China,2019,18(5):1147-1157.
[26] 顾咏梅,夏鑫慧,王宇婷,等.小黑杨MYB32基因生物信息学及应答盐胁迫分析[J].山西农业大学学报(自然科学版),2021,41(6):32-40.
[27] CHOU K C, SHEN H B. A new method for predicting the subcellular localization of eukaryotic proteins with both single and multiple sites:Euk-mPLoc 2.0[J].PLoS One,2010,5(4):e9931.
[28] GEOURJON C, DELÉAGE G. SOPMA:significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J].Bioinformatics,1995,11(6):681-684.
[29] POLEKSIC＇ A, GRAY P. Utilizing twilight zone sequence similarities to increase the accuracy of protein 3D structure comparison[C]//Proceedings of the 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics.Newport Beach California.New York, NY, USA:ACM,2014:297-303.
[30] NOWICK J S. Exploring beta-sheet structure and interactions with chemical model systems[J].Accounts of Chemical Research,2008,41(10):1319-1330.
[31] 彭茂潇,牛东红,李家乐.海洋无脊椎动物补体系统研究进展[J].海洋渔业,2016,38(3):320-328.
[32] 王镜岩.生物化学:上册[M].3版.北京:高等教育出版社,2002:181-189.
[33] 隋立军,刘卫东,李云峰,等.文蛤C1q基因的克隆与表达及其重组蛋白活性的研究[J].水产科学,2012,31(6):321-328.
[34] GERDOL M, VENIER P, PALLAVICINI A. The genome of the Pacific oyster Crassostrea gigas brings new insights on the massive expansion of the C1q gene family in Bivalvia[J].Developmental & Comparative Immunology,2015,49(1):59-71.
[35] 朱峰.仿刺参Apostichopus japonicus胚胎发育和主要系统的组织学研究[D].青岛:中国海洋大学,2009:14-19.
[36] 陈文博,许延,宋晓阳,等.刺参肠道菌群和消化酶在生态养殖中的作用及研究[J].水产养殖,2014,35(7):12-16.
[37] YANG A F, ZHOU Z C, HE C B, et al. Analysis of expressed sequence tags from body wall, intestine and respiratory tree of sea cucumber (Apostichopus japonicus)[J].Aquaculture,2009,296(3/4):193-199.
[38] 王洪迪, 崔军, 王秀利, 等. 刺参管足 miRNA 的生物信息学分析及表达的初步研究:“全球变化下的海洋与湖沼生态安全” 学术交流会论文摘要集[C].南京:中国海洋湖沼学会,2014.
[39] SUN L N, CHEN M Y, YANG H S, et al. Large scale gene expression profiling during intestine and body wall regeneration in the sea cucumber Apostichopus japonicus[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2011,6(2):195-205.
[40] 孙素凤.养殖刺参(Apostichopus japonicus)苗期细菌性疾病及其病原学初步研究[D].青岛:中国海洋大学,2006:1-2.
[41] 蒋经伟,丛聪,董颖,等.不同细菌刺激后仿刺参体腔液中免疫相关酶的应答变化[J].动物学杂志,2015,50(6):947-956.
[42] PRADO-ALVAREZ M, GESTAL C, NOVOA B, et al. Differentially expressed genes of the carpet shell clam Ruditapes decussatus against Perkinsus olseni[J].Fish & Shellfish Immunology,2009,26(1):72-83.
[43] GESTAL C, PALLAVICINI A, VENIER P, et al. MgC1q, a novel C1q-domain-containing protein involved in the immune response of Mytilus galloprovincialis[J].Developmental and Comparative Immunology,2010,34(9):926-934.
[44] ANGATA T. Molecular diversity and evolution of the Siglec family of cell-surface lectins[J].Molecular Diversity,2006,10(4):555-566. |
|
|
|
