|
|
|
| Changes in Muscle Quality and Physiochemical Properties of Grouper Hybrid Epinephelus fuscoguttatus ♂×E. lanceolatus ♀ during Keeping-alive with Water |
| WEN Jingjing1, WANG Lei1, WANG Jinxiang1, LI Xuepeng1, LI Jianrong1, DING Yuting2, XIE Jing3, WANG Yanbo4, QIN Xiaoming5 |
1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China;
2. College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
3. College of Food Science, Shanghai Ocean University, Shanghai 201306, China;
4. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;
5. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China |
|
|
|
|
Abstract The survival rate, body mass loss rate, general chemical composition and texture of muscle characteristics, muscle tissue morphology, serum biochemical indicators, fish gill Na+/K+-ATPase activity and tissue morphology were determined in pearl gentian grouper (Epinephelus fuscoguttatus♂×E. lanceolatus♀) with a body mass of (700±50) g placed in a 90 × 45 × 30 cm tank with continuous oxygenation for 0, 24, 48, 72, 96 and 120 h at a density of 100 g/L at a salinity of 24—26 and water temperature of (25±2) ℃to explore the changes in muscle quality and physiological and biochemical indicators at different water retention periods. The result showed that the survival rate of pearl gentian grouper was showed to be decreased, and body weight loss rate to be significantly increased (P<0.05), contents of crude fat and protein to be significantly decreased (P<0.05) with the time extended,while the hardness to be depressed gradually. With the extension of survival time, the morphology of muscles tissue was observed to be changed significantly, with increase in the gap between muscles fibers gradually, and the fractured structure of muscles tissue gradually. The activity of alanine aminotransferase and aspartate transaminase in fish blood was increased, while cortisol level went down firstly then up. There was decrease in Na+/K+-ATPase activity in gills and then increase. The lamellae became longer and thinner, accompanied by edema, and the whiteness of gills was increased significantly (P<0.05). The serum biochemical properties and the gills tissue morphology characteristics were expected to be potential indicators to characterize the of pearl gentian grouper during keeping-alive, which provided reference for the dynamic monitoring of the quality of live grouper during the live with water.
|
|
Received: 14 December 2021
|
|
|
|
|
|
[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2020中国渔业统计年鉴[M].北京:中国农业出版社,2020:21-23.
[2] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2019中国渔业统计年鉴[M].北京:中国农业出版社,2019:21-23.
[3] 王际英,张德瑞,马晶晶,等.珍珠龙胆石斑鱼肌肉营养成分分析与品质评价[J].海洋湖沼通报,2015(4):61-69.
[4] 朱乾峰,陈鹏文,范秀萍,等.珍珠龙胆石斑鱼低温有水保活条件优化[J].食品工业科技,2018,39(22):276-282.
[5] LI S, GUO H, CHEN Z Y, et al. Effects of acclimation temperature regime on the thermal tolerance, growth performance and gene expression of a cold-water fish, Schizothorax prenanti[J].Journal of Thermal Biology,2021,98:102918.
[6] 丁亚涛,汪之和,王林林,等.MS-222对鳊鱼麻醉保活运输效果的研究[J].水产科学,2019,38(3):296-304.
[7] 曹杰,谢晶.间氨基苯甲酸乙酯甲磺酸盐在大菱鲆模拟保活运输中的作用效果[J].食品科学,2021,42(23):246-253.
[8] 范秀萍,张家胜,郭侨玉,等.珍珠龙胆石斑鱼CO2麻醉无水保活效果的因素影响[J].广东海洋大学学报,2021,41(6):73-81.
[9] 邓甜甜,彭月欣,邓玛伊,等.鲫鱼食用酒精休眠法无水保活技术的研究[J].佛山科学技术学院学报(自然科学版),2019,37(4):21-25.
[10] 白婵,熊光权,黄敏,等.丁香酚麻醉斑点叉尾鮰在不同无水保活温度下的生理生化变化[J].渔业现代化,2019,46(1):60-66.
[11] WANG W S, ZHANG Y J, LIU Y, et al. Effects of waterless live transportation on survivability, physiological responses and flesh quality in Chinese farmed sturgeon (Acipenser schrenckii)[J].Aquaculture,2020,518:734834.
[12] FAN X P, QIN X M, ZHANG C H, et al. Metabolic and anti-oxidative stress responses to low temperatures during the waterless preservation of the hybrid grouper (Epinephelus fuscogutatus ♀ × Epinephelus lanceolatus ♂)[J].Aquaculture,2019,508:10-18.
[13] REFAEY M M, LI D P, TIAN X, et al. High stocking density alters growth performance, blood biochemistry, intestinal histology, and muscle quality of channel catfish Ictalurus punctatus[J].Aquaculture,2018,492:73-81.
[14] LONG L N, ZHANG H G, NI Q, et al. Effects of stocking density on growth, stress, and immune responses of juvenile Chinese sturgeon (Acipenser sinensis) in a recirculating aquaculture system[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2019,219:25-34.
[15] 袁小琛,陈范,王光毅,等.暂养时间对鳙肌肉品质和营养价值的影响[J].淡水渔业,2020,50(6):83-91.
[16] 廖明涛,刘书臣,林森森,等.金枪鱼鱼肉色泽评定方法的研究进展[J].食品与发酵工业,2013,39(6):135-140.
[17] 丁立云,陈文静,饶毅,等.饥饿胁迫对彭泽鲫幼鱼生长、体组成、消化酶活性及抗氧化性的影响[J].河南农业科学,2019,48(1):141-145.
[18] 覃川杰,邵婷,杨洁萍,等.饥饿胁迫对瓦氏黄颡鱼脂肪代谢的影响[J].水生生物学报,2015,39(1):58-65.
[19] 王双健,丁玉惠,江茂旺,等.饥饿时间对管角螺生长、生化组成、消化酶活性和抗氧化指标的影响[J].动物营养学报,2018,30(1):138-146.
[20] 高捷,刘红英,齐凤生,等.缢蛏保活过程中糖原变化的研究[J].食品工业科技,2011,32(5):361-362.
[21] 朱站英,华雪铭,于宁,等.草鱼蛋白质和脂肪代谢对饥饿胁迫的响应[J].水产学报,2012,36(5):756-763.
[22] LIU J, ARNER A, PUOLANNE E, et al. On the water-holding of myofibrils:effect of sarcoplasmic protein denaturation[J].Meat Science,2016,119:32-40.
[23] 吴朝朝,陈丽丽,袁美兰,等.短期暂养对鲫鱼肉品质影响的研究[J].食品工业科技,2015,36(15):334-337.
[24] LIN W L, ZENG Q X, ZHU Z W. Different changes in mastication between crisp grass carp (Ctenopharyngodon idellus C.et V) and grass carp (Ctenopharyngodon idellus) after heating:the relationship between texture and ultrastructure in muscle tissue[J].Food Research International,2009,42(2):271-278.
[25] 李宝山,冷向军,李小勤,等.投饲蚕豆对草鱼生长和肌肉品质的影响[J].中国水产科学,2008,15(6):1042-1049.
[26] 林婉玲,杨贤庆,李来好,等.脆肉鲩质构与感官评价的相关性研究[J].现代食品科技,2013,29(1):1-7.
[27] 刘丽丹,周瑶,孙庆业.煤矸石对鲫鱼体内微量元素含量及组织结构的影响[J].生态环境学报,2021,30(4):798-804.
[28] AUTHMAN M M N, ABBAS W T, GAAFAR A Y. Metals concentrations in Nile tilapia Oreochromis niloticus (Linnaeus, 1758) from illegal fish farm in Al-Minufiya Province, Egypt, and their effects on some tissues structures[J].Ecotoxicology and Environmental Safety,2012,84:163-172.
[29] 何伟,曹振东,付世建.温度和低氧对白鲢乳酸与糖水平的影响[J].重庆师范大学学报(自然科学版),2013,30(5):27-31.
[30] 苏艳莉,戈贤平,孙盛明,等.高温季节饥饿后补偿摄食对团头鲂幼鱼生长性能、血清生化指标、肠道消化酶活性及肝脏抗氧化酶活性的影响[J].动物营养学报,2017,29(11):4198-4206.
[31] 王利娟.大口黑鲈保活运输的研究[D].上海:上海海洋大学,2015:25-27.
[32] 朱乾峰.珍珠龙胆石斑鱼低温保活运输技术研究[D].湛江:广东海洋大学,2018:14-15.
[33] 李勇男,刘海英,苏从毅.鱼类的运输应激反应诱发因素、影响及缓解措施[J].食品工业科技,2015,36(23):391-394.
[34] 夏媛香.饥饿影响对南方鲇和草鱼生长及能量代谢的比较研究[D].重庆:西南大学,2018:9.
[35] 周翠平.罗非鱼二氧化碳麻醉无水保活技术研究[D].湛江:广东海洋大学,2015:4-5.
[36] 丁亚涛.鳊鱼保活运输技术的研究[D].上海:上海海洋大学,2019:18-21.
[37] EVANS D H, PIERMARINI P M, CHOE K P. The multifunctional fish gill:dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste[J].Physiological Reviews,2005,85(1):97-177.
[38] 柳旭东,王际英,张利民,等.影响水产动物Na+-K+-ATP酶活力的水环境因素[J].水产科学,2009,28(3):171-175.
[39] ESBAUGH A J, BRIX K V, GROSELL M. Na+ K+ ATPase isoform switching in zebrafish during transition to dilute freshwater habitats[J].Proceedings.Biological Sciences,2019,286(1903):20190630.
[40] 苏慧,区又君,李加儿,等.饥饿对卵形鲳鲹幼鱼不同组织抗氧化能力、Na+/K+-ATP酶活力和鱼体生化组成的影响[J].南方水产科学,2012,8(6):28-36.
[41] HOSEINI S M, KHALILI M, RAJABIESTERABADI H, et al. Effects of dietary monoterpene, myrcene, administration on immune-and health-related genes expression in common carp gill following exposure to copper sulfate[J].Fish & Shellfish Immunology,2020,98:438-445.
[42] 刘娟娟,王京树,赵繤,等.不同盐度和驯养时间中华鲟子二代幼鱼鳃的显微结构变化[J].水生态学杂志,2015,36(1):60-65.
[43] 温久福,蓝军南,曹明,等.盐度对花鲈幼鱼鳃、脾及肌肉组织结构的影响[J].渔业科学进展,2020,41(1):112-118. |
|
|
|
