不同营养水平对鳜幼鱼糖异生和糖原分解的影响

doi:10.16378/j.cnki.1003-1111.22088

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Abstract In order to explore the effects of three nutrient levels of normal feeding, glucose injection and starvation on the endogenous glucose production pathway of mandarin fish (Siniperca chuatsi), mandarin fish with body weight of (36.70±0.52) g were reared in cages of 1 m×1 m×1 m at stocking density of 25, 25 and 15 fish per box at water temperature of (23.0±0.5) °C, respectively, and were fed live grass carp Ctenopharyngodon idella with body weight of (8.15±0.16) g fingerlings (normal feeding group), 1 mg/g body weight intraperitoneal injection of glucose (glucose group) and starvation for 15 days (starvation group). Blood glucose and liver glycogen contents, insulin level, glucagon level, and activities of key enzymes in gluconeogenesis, including phosphoenolpyruvate carboxykinase (PEPCK), fructose-1,6-bisphosphatase (FBPase) , and glucose-6-phosphatase(G6Pase)were measured 1, 2, 4, 6, 12 and 16 h after feeding, 1, 2, 4, 6, 8, 12, and 24 h after injection, and 5, 10 and 15 days after starvation, and the relative mRNA expression levels was detected in each enzyme gene using real-time PCR. The results showed that blood glucose and liver glycogen contents and insulin level were significantly increased in the normal feeding group, and glucagon was significantly decreased, without significant change in the activity of key enzymes and inhibition of the activities of GPase, FBPase and GPase in gluconeogenesis. There were persistent hyperglycemia, and inhibition of PEPCK and the GPase activities and the relative expression levels of GPase in glucose injection group, without inhibition of the FBPase and G6Pase activities, and without significant changes in relative expression levels of PEPCK, FBPase, and G6Pase genes. The mandarin fish in starvation group had significant decrease in the blood glucose content in 10 days, and significant increase in glucagon content for 5 days, mainly relying on glycogenolysis to generate glucose from significantly elevated GPase activity during pre-and mid-stage (5 d and 10 d) of starvation. There were significant increase in relative expression levels of GPase, PEPCK, and G6Pase genes, thus glucose was mainly generated by gluconeogenesis at 15 day starvation. It was found that the level of blood glucose was significantly increased in the normal feeding group, and that the glycogenolysis pathway was significantly inhibited. The increase in 0—6 h blood glucose content was speculated to be caused by food digestion and absorption, with the peak of “hyperglycemia” at 6 h, and 6—16 h food digestion and absorption continue, after glucose absorption, glycogen synthesis was significantly improved, thus maintaining blood glucose balance. Due to high blood glucose levels, at the same time, glycogenolysis was inhibited (GPase activity decreased 0—12 h). The highest blood glucose level at 6 h reflected the time that the mandarin fish maintained "blood glucose balance". If the blood glucose level continued to rise, it caused physiological abnormalities in the body. The levels of blood glucose had different effects on gluconeogenesis and glycogenolysis pathways of mandarin fish. The gluconeogenesis and glycogenolysis pathways were regulated by the level of blood glucose.

Key words： Siniperca chuatsi level of blood glucose glucose metabolism pathway

Received: 08 October 2022

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	MA Chenxi
	ZHAO Jinliang
	SONG Yindu
	ZHAO Liangliang

Cite this article:

MA Chenxi,ZHAO Jinliang,SONG Yindu, et al. Effects of Different Nutrient Levels on Gluconeogenesis and Glycogenolysis of Juvenile Mandarin Fish Siniperca chuatsi[J]. Fisheries Science, 2024, 43(5): 707-716.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22088 OR https://www.shchkx.com/EN/Y2024/V43/I5/707

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