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| Denitrifying Microorganisms on Suspended Carrier Biofilm in a Recirculating Aquaculture System of Pacific White Shrimp Litopenaeus vannamei |
| LI Yayuan1,2,3, ZHANG Wang1,2,3, LIU Xingguo2,3, XIAO Shuwen1,2,3, JIANG Haixin4, LU Shimin2,3 |
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2. Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China;
3. Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Shanghai 200092, China;
4. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China |
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Abstract To understand the nitrate reduction potential and nitrogen metabolism pathway character of moving bed bioreactor in a Pacific white shrimp Litopenaeus vannamei in a recirculating aquaculture system(RAS), the microbial community structure and denitrification efficiency of biofilms were analyzed on the surface of suspended carriers. Illumina sequencing results revealed that Proteobacteria(20.31%—25.36%) and Bacteroidetes(14.42%—24.28%) were dominant in all samples. At the genus level, aerobic denitrifiers such as Rhodococcus (0.28%—0.97%), Paracoccus (0.50%—0.99%) and Pseudomonas (0.15%—0.30%) were primarily responsible for nitrate reduction. Furthermore, qRT-PCR detection showed that the denitrification functional gene nirK was more abundant than that of nirS and nosZ, so the predominant denitrification bacteria were of the nirK-type.The prediction results from functional annotation of prokaryotic taxa (FAPROTAX) confirmed the presence of active heterotrophic denitrification in biofilms, the denitrifying bacteria were mainly competitive with each other and with the dominant biofilm bacteria,possibly due to the limited carbon sources. The anaerobic nitrogen removal tests showed that there was nitrate removal efficiency of up to 98.07%, without adding any carbon source. Based on the characteristics of nitrogen metabolism pathway, the partial denitrification-anammox process for shrimp aquaculture wastewater nitrogen removal was further proposed. The finding provides novel insights into comprehending of nitrogen metabolic pathways in RAS and future management, utilization of endogenous carbon sources in the biofilms, and potential denitrification functions.
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Received: 09 February 2023
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