耐低温降解菌<i>Bacillus mobilis</i> 10的生物学特性及其对海参养殖水质的净化作用

doi:10.16378/j.cnki.1003-1111.23030

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Abstract A bacterium strain collected from the coastal soil of Qingdao was isolated and purified after 48 h of culture at 15 °C with 7 media, and was identified as Bacillus mobilis by morphological observation, physicochemical characteristics and 16S rRNA gene sequence analysis. The strain was inoculated into LB liquid culture at 5, 28 and 37 °C and 0, 2%, 4%, 6% and 8% of NaCl to explore the temperature and salinity tolerance of the B. mobilis, and the strain was sprayed once every 3 days in a sea cucumber Apostichopus japanicus seedling pond with a final concentration of about 1.0×10⁴ cfu/mL to purify the water quality in the sea cucumber culture pond, so as to realize the industrialization of this strain.The transparent circle method showed that B. mobilis 10 produced protease, amylase and cellulase activities at low temperature conditions (15 °C), with protease activity of 9.05 U/mL after 56 h of fermentation and NaCl tolerance of up to 8%.The test of degradation ability of the strain to dissolved substances showed that the degradation rate in the feed culture medium was observed to be (36.72±0.53)% in protein and (22.80±0.81)% in (chemical oxygen demand) COD using sea cucumber feed culture medium, 8 h after inoculation. Pilot-scale (50 L) fermentation techniques was established to microbial preparation, and applied in the sea cucumber breeding. Dynamic monitoring of the bacterial activity of B. mobilis 10 in sea cucumber culture ponds for 8 days revealed that both the contents of protein and COD in sea cucumber ponds were significantly reduced by sprinkling the bacterial solution with the final concentration of 1.0×10⁴ cfu/mL. The findings had exemplified the successful exploration case regarding the development of microbial products for use in aquaculture practice.

Key words： psychrophile Bacillus mobilis biological characteristics sea cucumber culture water quality purification

Received: 26 April 2023

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	TANG Wei
	WANG Qi
	ZHANG Jun
	LI Jiaxin
	XING Wei
	HE Zengguo

Cite this article:

TANG Wei,WANG Qi,ZHANG Jun, et al. Biological Characteristics and Application in Water Purification in Sea Cucumber Culture Ponds in Cryotolerant Degrading Bacterium Strain Bacillus mobilis 10[J]. Fisheries Science, 2024, 43(6): 885-893.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23030 OR https://www.shchkx.com/EN/Y2024/V43/I6/885

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