两种微藻对凡纳滨对虾养殖系统水质与生物膜菌群结构的影响

doi:10.16378/j.cnki.1003-1111.24037

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (14386 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to investigate the effects of addition of green algae Chlorella pyrenoidosa and Haematococcus pluvialis on water quality, growth, biofilm bacterial community structure, and diversity of the Pacific white shrimp Litopenaeus vannamei in an internal circulation aquaculture system, a hydrophilic polyurethane gel filler with hanging film was loaded into a mesh bag, placed in an 80 L glass tank at 5% (V/V), with a salinity of 15 artificial seawater, and 64 juvenile shrimp (800 individuals/m³) with body length of (0.8±0.1) cm were stocked, and reared for 84 d and 10 mL of C. pyrenoidosa and H. pluvialis were added every 3 days, respectively, and no algae were added to the control group at water temperature of 26—28 ℃. Wash the hydrophilic polyurethane gel filler every 7 days and replenish it regularly. The results showed that the addition of microalgae led to reduce contents of nitrate nitrogen (NO^-₃-N) and total phosphorus (TP) in the aquaculture system, with decrease in NO_3--N and TP by 13.0% and 17.7% in C. pyrenoidosa group and 6.3% and 29.7% H. pluvialis group. However, there were variations in ammonia nitrogen (TAN)and nitrite nitrogen (NO^-₂-N) performance: decrease in concentration in H. pluvialis group and increase in C. pyrenoidosa group in the aquaculture system. The body length, body weight, average daily growth rate (ADG), and feed utilization efficiency were improved in the shrimp exposed to addition of microalgae, without significant effect on shrimp survival rate. Body weight was found to be increased in the shrimp by 15.0% in C. pyrenoidosa group and 11.8% in H. pluvialis group.Analysis of bacterial community structure and diversity revealed that the relative abundances of Proteobacteria and Bacteroidetes were decreased in C. pyrenoidosa system and the relative abundance of Acidobacteria was increased; relative abundance of Bacteroidetes was increased in H. pluvialis system while Acidobacteria and Proteobacteria were decreased instead. Additionally, the relative abundance of nitrifying bacteria was shown to be increased in the added microalgae culture systems, while the relative abundance of denitrifying bacteria to be decreased.

Key words： Litopenaeus vannamei microalga water quality bacterial community structure of biofilm

Received: 11 March 2024

S968.22

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LYU Libin
	LIU Chao
	SUN Mingzhu
	XU Ailing
	SONG Zhiwen

Cite this article:

LYU Libin,LIU Chao,SUN Mingzhu, et al. Effects of Two Species of Green Microalgae on Water Quality and Biofilm Flora Structure in Aquaculture Systems of Pacific White Shrimp Litopenaeus vannamei[J]. Fisheries Science, 2025, 44(2): 223-234.

URL:

https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24037 OR https://www.shchkx.com/EN/Y2025/V44/I2/223

[1] ALEISSA A, CHEN P, BROWN P B, et al. Effects of feed formula and farming system on the environmental performance of shrimp production chain from a life cycle perspective[J] . Journal of Industrial Ecology,2022,26(6):2006-2019.
[2] MEDINA-FÉLIX D, LÓPEZ-ELÍAS J A, MARTÍNEZ-CÓRDOVA L R, et al. Evaluation of the productive and physiological responses of Litopenaeus vannamei infected with WSSV and fed diets enriched with Dunaliella sp.[J] . Journal of Invertebrate Pathology,2014,117:9-12.
[3] KANG Y H, KIM S, CHOI S K, et al. A comparison of the bioremediation potential of five seaweed species in an integrated fish-seaweed aquaculture system:implication for a multi-species seaweed culture[J] . Reviews in Aquaculture,2021,13(1):353-364.
[4] 俞建中,梁欣欣,魏东,等.三种饵料微藻在凡纳滨对虾育苗中的饲喂效果评价[J] .现代食品科技,2013,29(4):762-767.
[5] 沈南南,李纯厚,贾晓平,等.小球藻与芽孢杆菌对对虾养殖水质调控作用的研究[J] .海洋水产研究,2008,29(2):48-52.
[6] 陈春云,庄源益,方圣琼.小球藻对养殖废水中N、P的去除研究[J] .海洋环境科学,2009,28(1):9-11.
[7] 吴长斌,张佳峰,翟兴文.氮源对雨生红球藻和扁藻生长的影响[J] .河北渔业,2003(2):24-25.
[8] 张怀瑾,张晶晶,周进,等.城市生活污水用于培养雨生红球藻的研究[J] .中国生物工程杂志,2016,36(11):39-47.
[9] 李玥睿,高金伟,张文慧,等.蛋白核小球藻粉和雨生红球藻粉对中华绒螯蟹幼蟹生长和生理生化指标的影响[J] .饲料研究,2022,45(20):49-54.
[10] CHIEN Y H, SHIAU W C. The effects of dietary supplementation of algae and synthetic astaxanthin on body astaxanthin, survival, growth, and low dissolved oxygen stress resistance of kuruma prawn, Marsupenaeus japonicus Bate[J] . Journal of Experimental Marine Biology and Ecology,2005,318(2):201-211.
[11] ZHANG J, LIU Y J, TIAN L X, et al. Effects of dietary astaxanthin on growth, antioxidant capacity and gene expression in Pacific white shrimp Litopenaeus vannamei[J] . Aquaculture Nutrition,2013,19(6):917-927.
[12] LONG X W, WU X G, ZHAO L, et al. Effects of dietary supplementation with Haematococcus pluvialis cell powder on coloration, ovarian development and antioxidation capacity of adult female Chinese mitten crab, Eriocheir sinensis[J] . Aquaculture,2017,473:545-553.
[13] AUDELO-NARANJO J M, MARTINEZ-CORDOVA L R, GOMEZ-JIMENEZ S, et al. Intensive culture of Litopenaeus vannamei without water exchange and with an artificial substrate[J] . Hydrobiológica, 2012,22(1):1-7.
[14] FERREIRA L M H, LARA G, WASIELESKY JR W, et al. Biofilm versus biofloc:are artificial substrates for biofilm production necessary in the BFT system?[J] . Aquaculture International,2016,24(4):921-930.
[15] 杨帆,邓学智,刘佳,等.4种矿物质载体对硝化微生物富集培养的影响[J] .环境科学与技术,2020,43(10):27-33.
[16] SESUK T, POWTONGSOOK S, NOOTONG K. Inorganic nitrogen control in a novel zero-water exchanged aquaculture system integrated with airlift-submerged fibrous nitrifying biofilters[J] . Bioresource Technology,2009,100(6):2088-2094.
[17] SCHVEITZER R, ARANTES R, BALOI M F, et al. Use of artificial substrates in the culture of Litopenaeus vannamei (Biofloc System) at different stocking densities:Effects on microbial activity, water quality and production rates[J] . Aquacultural Engineering,2013,54:93-103.
[18] SATANWAT P, TRAN T P, HIRAKATA Y, et al. Use of an internal fibrous biofilter for intermittent nitrification and denitrification treatments in a zero-discharge shrimp culture tank[J] . Aquacultural Engineering,2020,88:102041.
[19] OWATARI M S, JESUS G F A, DE MELO FILHO M E S, et al. Synthetic fibre as biological support in freshwater recirculating aquaculture systems (RAS)[J] . Aquacultural Engineering,2018,82:56-62.
[20] WANG Y, SUN M Z, TANG Y Z, et al. Effects of Haematococcus pluvialis on the water quality and performance of Litopenaeus vannamei using artificial substrates and water exchange systems[J] . Aquaculture International,2022,30(4):1779-1797.
[21] SONG Z W, LIU C, LUAN Y Z, et al. Effect of zero water exchange systems for Litopenaeus vannamei using sponge biocarriers to control inorganic nitrogen and suspended solids simultaneously[J] . Sustainability,2023,15(2):1271.
[22] LUAN Y Z, WANG Y, LIU C, et al. Effects of potassium monopersulfate on nitrification activity and bacterial community structure of sponge biocarrier biofilm in Litopenaeus vannamei aquaculture system[J] . Environmental Technology,2024,45(17):3354-3366.
[23] 国家环境保护总局. 水和废水监测分析方法[M] .4版.北京:中国环境科学出版社,2002.
[24] VALENCIA-CASTAÑEDA G, FRÍAS-ESPERICUETA M G, VANEGAS-PÉREZ R C,et al. Acute toxicity of ammonia, nitrite and nitrate to shrimp Litopenaeus vannamei postlarvae in low-salinity water[J] . Bulletin of Environmental Contamination and Toxicology,2018,101(2):229-234.
[25] 魏泰莉,余瑞兰,聂湘平,等.水中亚硝酸盐对彭泽鲫血红蛋白及高铁血红蛋白的影响[J] .大连水产学院学报,2001,16(1):67-71.
[26] WU D X, CHENG M L, ZHAO S M, et al. Algal growth enhances light-mediated limitation of bacterial nitrification in an aquaculture system[J] . Water, Air, & Soil Pollution,2020,231(2):73.
[27] LI H Y, HOLLSTEIN M, PODDER A, et al. Cyanotoxin impact on microbial-mediated nitrogen transformations at the interface of sediment-water column in surface water bodies[J] . Environmental Pollution,2020,266:115283.
[28] GAO P T, GUO L, ZHAO Y G, et al. Enhancing microalgae growth and product accumulation with carbon source regulation:new perspective for the coordination between photosynthesis and aerobic respiration[J] . Chemosphere,2021,278:130435.
[29] GUPTA P L, CHOI H J, LEE S M. Enhanced nutrient removal from municipal wastewater assisted by mixotrophic microalgal cultivation using glycerol[J] . Environmental Science and Pollution Research International,2016,23(10):10114-10123.
[30] MALLICK N, RAI L C. Influence of culture density, pH, organic acids and divalent cations on the removal of nutrients and metals by immobilized Anabaena doliolum and Chlorella vulgaris[J] . World Journal of Microbiology and Biotechnology,1993,9(2):196-201.
[31] LIM K C, YUSOFF F M, SHARIFF M, et al. Astaxanthin as feed supplement in aquatic animals[J] . Reviews in Aquaculture,2018,10(3):738-773.
[32] CUZON G, LAWRENCE A, GAXIOLA G, et al. Nutrition of Litopenaeus vannamei reared in tanks or in ponds[J] . Aquaculture,2004,235(1/2/3/4):513-551.
[33] FLORES-VALENZUELA E, MIRANDA-BAEZA A, RIVAS-VEGA M E, et al. Water quality and productive response of Litopenaeus vannamei reared in biofloc with addition of commercial strains of nitrifying bacteria and Lactobacillus rhamnosus[J] . Aquaculture,2021,542:736869.
[34] 曲疆奇,张清靖,杨浩辰,等.养殖尾水处理系统填料生物膜氮循环微生物功能特征[J] .水生态学杂志,2023,44(2):104-113.
[35] HUANG F, XUE D F, LI Z, et al. Evaluation of dietary Haematococcus pluvialis in pilot-scale farming of Litopenaeus vannamei:effects on production performance, physiological health, bacterial communities, and eukaryotic plankton[J] . Aquaculture,2023,563:738858.
[36] REJI L T, ZHANG X N. Genome-resolved metagenomics informs the functional ecology of uncultured acidobacteria in redox oscillated Sphagnum peat[J] . mSystems,2022,7(5):e0005522.
[37] SPEIRS L B M, RICE D T F, PETROVSKI S, et al. The phylogeny, biodiversity, and ecology of the Chloroflexi in activated sludge[J] . Frontiers in Microbiology,2019,10:2015.
[38] SOROKIN D Y, VEJMELKOVA D, LÜCKER S, et al. Nitrolancea hollandica gen. nov., sp. nov., a chemolithoautotrophic nitrite-oxidizing bacterium isolated from a bioreactor belonging to the Phylum Chloroflexi[J] . International Journal of Systematic and Evolutionary Microbiology,2014,64(Pt 6):1859-1865.
[39] SOROKIN D Y, LÜCKER S, VEJMELKOVA D, et al. Nitrification expanded:discovery, physiology and genomics of a nitrite-oxidizing bacterium from the Phylum Chloroflexi[J] . The ISME Journal,2012,6(12):2245-2256.
[40] ELBON C E, LECLEIR G R, TUTTLE M J, et al. Microbiomes and Planctomycete diversity in large-scale aquaria habitats[J] . PLoS One,2022,17(5):e0267881.
[41] DEL'DUCA A, CESAR D E, FREATO T A, et al. Variability of the nitrifying bacteria in the biofilm and water column of a recirculating aquaculture system for tilapia (Oreochromis niloticus) production[J] . Aquaculture Research,2019,50(9):2537-2544.
[42] MOSCHOS S, KORMAS K A, KARAYANNI H. Prokaryotic diversity in marine and freshwater recirculating aquaculture systems[J] . Reviews in Aquaculture, 2022,14(4):1861-1886.
[43] CAO X Y, LI H, ZHOU Y Y, et al. The shift of mutualistic relationships among algae, free-living and attached bacteria through different nutrient addition mode:a mesocosm study[J] . Journal of Freshwater Ecology,2020,35(1):535-548.
[44] VICO P, IRIARTE A, BONILLA S, et al. Metagenomic analysis of Raphidiopsisraciborskii microbiome:beyond the individual[J] . Biodiversity Data Journal,2021,9:e72514.
[45] NAGANUMA T, FUKAI I, MURAKAMI Y, et al. Effect of ultraviolet radiation on the bioavailability of marine diatom-derived low-molecular-weight dissolved organic matter[J] . Aquatic Ecosystem Health and Management,2000,3(1):163-166.
[46] FUKUNAGA Y, KURAHASHI M, YANAGI K, et al. Acanthopleuribacter pedis gen. nov., sp. nov., a marine bacterium isolated from a Chiton, and description of Acanthopleuribacteraceae fam. nov., Acanthopleuribacterales ord. nov., Holophagaceae fam. nov. , Holophagales ord. nov. and Holophagae classis nov. in the Phylum ‘Acidobacteria’[J] . International Journal of Systematic and Evolutionary Microbiology,2008,58(Pt 11):2597-2601.
[47] JIANG X, REN C H, HU C Q, et al. Isolation and algicidal characterization of Bowmanella denitrificans S088 against Chlorella vulgaris[J] . World Journal of Microbiology & Biotechnology,2014,30(2):621-629.
[48] KULICHEVSKAYA I S, IVANOVA A O, BELOVA S E, et al. Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands[J] . International Journal of Systematic and Evolutionary Microbiology,2007,57(Pt 11):2680-2687.