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| Effects of Three Microalgal Species and Densities on Physiological Metabolism, and Carbon and Nitrogen Budgets of Pearl Oyster Pinctada fucata martensii |
| REN Peng1, ZHANG Xingzhi2, LIU Jingcan1, LYU Shuguo3, WANG Aimin1,3, LIU Chunsheng1 |
1. School of Marine Biology and Fisheries, Hainan University, Haikou 570228, China;
2. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning 530021, China;
3. Hainan International Blue Carbon Research Center, Haikou 571126, China |
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Abstract To elucidate the effects of different microalgae species and concentrations on physiological metabolism and carbon and nitrogen budget of pearl oyster Pinctada fucata martensii, the pearl oyster with shell length of (55.06±1.22) mm and wet body weight of (23.26±1.12) g were reared in plastic barrels of 200 L sand-filtered seawater, and was fed microalgae Isochrysis galbana, Chaetoceros muelleri and Platymonas subcordiformis at concentrations of each alga 2.2, 4.4 and 6.6 mg/L (dry weight), respectively, at water temperature of (26.0±0.5) °C. The physiological and metabolic indices including food ingestion rate, oxygen consumption rate, ammonia excretion rate, fecal excretion rate and calcification rate of Pinctada martensii under the interaction of microalga species and concentrations were investigated, and then carbon and nitrogen budget of the pearl oyster was calculated. The results showed the food ingestion rate of the pearl oyster was significantly influenced by microalgal species and concentrations (P<0.05), with from 13.36 to 39.14 mg/(g·h). In the I. galbana group, the pearl oyster and food ingestion rates in a trend of initially increasing and then decreasing with food concentration, while in the other two treatment groups, the food ingestion rates were found to be increased with food concentration. The respiration and ammonia excretion rates of the pearl oyster were significantly influenced by microalgal species, with lower respiration rates [0.63—1.34 mg/(g·h)]in P. subcordiformis group, and higher ammonia excretion rates [0.44—0.71 mg/(g·h)]in C. muelleri group. Growth carbon was significantly influenced by microalgal species and concentration in carbon budget of the pearl oyster. The proportion of growth carbon was shown to be increased with the increase in food concentration under the experimental conditions, with the maximal proportion of growth carbon in the I. galbana group, followed by the C. muelleri group, and the P. subcordiformis group at the same microalgal concentration. The pearl oyster has a higher proportion of excreted nitrogen (36.75%—81.63%) and a lower proportion of growth nitrogen (-26.91%—29.48%) in nitrogen budget under low microalgal concentration condition (2.2 mg/L). At higher microalgal concentrations (4.4 and 6.6 mg/L), however, the maximal proportion of growth nitrogen was observed in the I. galbana group, followed by the P. subcordiformis group, and the lowest in the C. muelleri group. The parameters of various physiological metabolisms and carbon and nitrogen budgets were quantified in the pearl oyster under different microalgal species and concentrations. The findings can provide reference data for enhancing the farming techniques of bivalves and investigating their ecological adaptability mechanisms.
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Received: 18 June 2024
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