不同生长速率单环刺螠幼体肠道菌群差异分析

doi:10.16378/j.cnki.1003-1111.24045

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Abstract In order to explore the relationship between growth difference of juvenile echiura worm Urechisunicinctus and its intestinal microbiota with different growth rates, the composition and changes in bacterial flora were analyzed in the intestines especially dissected of the juvenile echiura worm anesthesized by MS-222 with body weight of greater than 700 mg in fast-growth group (FG group) and with body weight of less than 300 mg in slow-growth group (SG group) sampled in three 100 cm² substrate regions in the same culture pond by 16S rRNA high-throughput sequencing. The results showed that greater diversity of intestinal flora species was found in the juvenile echiura worm in FG group than that in FG group, with dominant bacteria in Proteobacteria and Firmicutes in the intestine. There was the maximal relative abundance of Ralstonia in FG group and significantly higher than that in SG group, while there was the maximal abundance of Candidatus_hepatoplasma in SG group and significantly higher than that in FG group.LEfSe analysis revealed that more Pseudomonas, which was speculated to promote individual growth, was observed in FG group, with more Candidatus_hepatoplasma in SG group, or the marker genus with too slow growth rate. Functional prediction showed that the relative abundance of bacteria involved in cellular processes and signaling was significantly higher in FG group than that in SG group, while the relative abundance of microorganisms involved in cell growth and death function groups accounted for a significant advantage in SG group, indicating that there was a correlation between the growth rate of echiura worm and the intestinal flora. The findings can provide theoretical guidance for the healthy breeding and efficient cultivation of echiura worm.

Key words： Urechis unicinctus growth rate intestinal flora 16S rRNA

Received: 27 March 2024

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	DONG Haomiao
	WANG Fangyi
	XU Dong
	JIAO Xudong
	WANG Weizhong

Cite this article:

DONG Haomiao,WANG Fangyi,XU Dong, et al. Difference Analysis in Intestinal Flora of Juvenile Echiura Worm Urechis unicinctus with Different Growth Rates[J]. Fisheries Science, 2025, 44(2): 303-312.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24045 OR https://www.shchkx.com/EN/Y2025/V44/I2/303

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