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| Effect of Tussah Immunoreactive Substances on Growth and Immunity of Sea Urchin Strongylocentrotus intermedius |
| CHEN Xinghe1,2, JIA Xinyue1,2, WEN Zhixin1,2, LI Yajie1,2, WANG Dong3, DU Xingfan1,2 |
1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China;
2. Liaoning Professional Technology Innovation Center of Further Processing and Utilization of Tussah Resources, Dalian 116024, China;
3. Dalian Scitech Biotechnology Co., Ltd, Dalian 116600, China |
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Abstract To investigate the effect of tussah immunoreactive substances (TIS) on growth and immune function of sea urchin Strongylocentrotus intermedius, the sea urchin with initial body weight of (26.7±0.2) g were reared in a 97 L tank at stocking density of 30 individuals per tank, and fed the diets containing 0% (control group), 0.5, 1.0, and 2.0% of TIS for 60 days. At the end of the experiment, growth performance and immune indices were measured in body coelomic fluid. The results showed that the specific growth rate and shell diameter growth rate were found to be increased in the sea urchins in the 0.5% and 1.0% TIS-fed groups compared to the control group, without significant differences, and significant increase in the gonado-somatic index (P<0.05). The total amount of flavor amino acids was shown to be increased by 5.4% in the gonads of sea urchins in the 1.0% TIS group compared to the control group. Moreover, there was more than 10% increase in the content of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in unsaturated fatty acids. The sea urchin had significant activities of lysozyme and superoxide dismutase in each TIS-supplemented group as well as the activity of catalase in the sea urchin gonads in the 0.5% dose group (P<0.05). In summary, diet supplemented with 0.5%—1.0% TIS can lead to enhance the growth and improve the immune response of the sea urchin. These findings offer a theoretical framework for integrating TIS into sea urchin formula feed.
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Received: 21 November 2023
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