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| Analysis of Feeding Habits of Mystus guttatus Based on DNA Macro Barcode Technology |
| MENG Qingmi1,2, YANG Li 1,2, MA Lan1,2, YAO Junjie1,2, MO Xianyi3, CHEN Jiwei3 |
1. Research Center of Fishery Resources and Environment, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics and Breeding and Reproduction of Plateau and Mountain Animals, Guizhou University, Guiyang 550025, China;
3. Luodian County Aquaculture Development Center, Qiannan 550100, China |
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Abstract In order to understand the feeding habits of Mystus guttatus, food composition and feeding selectivity were analyzed in 9 samples of M. guttatus randomly collected from the river channel in Luodian County, Guizhou Province and in water samples on July 18, 2023 by DNA metabarcoding analysis technology, using 18S rDNA V4 region as a molecular marker for amplification. The results showed that 14 phyla were identified in the gastric contents of the M. guttatus, including Arthropoda, Chordata, Rotifera, Chlorophyta, Bacillariophyta, Ciliophora, Cercozoa, Streptophyta, Imbricatea, Platyhelminthes, Nematoda, Gastrotricha, Annelida and Haptista, with the maximal relative abundance for Arthropoda in the gastric contents of the Mystus guttatus, followed by the Chordata. However, the maximal relative abundance was found in Barbus in the Chordata, followed by Brachionus in the Rotifera and Sinodiaptomus in the Arthropoda based on genus level. The M. guttatus had the maximal active feeding selectivity towards the Chordata, with high feeding preference for the phyla of Cercozoa, Rotifera, and Bacillariophyta, and random feeding in the Ciliophora, Arthropoda, and Chlorophyta, indicating that the M. guttatus is a benthic carnivorous fish, and that main food source comes from the Chordata and Arthropoda. From a genus perspective, the main food sources were showed to be Barbus in fish in the Chordata and the crustaceans in the Arthropoda, including Sinodiaptomus and Macrobrachium. Arthropoda was a readily available food and Chordata was a favorite food. Therefore, the selection of food habits by M. guttatus was influenced by two factors: the availability and preference of food in the water environment.
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Received: 10 March 2024
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