|
|
|
| Growth and Quality Traits of Low-Copper Strains of Fujian Oyster |
| TANG Hongning1, ZHANG Hong1, QUE Huayong1, GONG Shihai2, SHI Bo1 |
1. State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, China;
2. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China |
|
|
|
|
Abstract In order to investigate the quality traits including growth traits, mineral and glycogen contents of Fujian oyster Crassostrea angulata, three consecutive generations of population breeding were bred, and the female and male low-copper content breeding oyster lines were selected by 20% selection pressure, and the low-copper F3 generation breeding lines were obtained after selective breeding, and then the Fujian oyster in self-propagation group and the ordinary breeding population of the oyster were ordinarily conducted in the oyster culture area of Zhao'an Bay, Fujian Province. The attached and metamorphosis juveniles were transferred to Hui'an area of Quanzhou for further culture for 3 months, and then transferred to cage culture in Liu'ao and Lianjiang areas of Fujian Province, and compared with ordinary breeding groups. The growth traits and quality traits were detected including contents of glycogen, minerals, and protein in 6, 9 and 12 months old Fujian oyster. The results showed that the low-copper and common Fujian oyster had copper (Cu) content from 114.24 to 750.50 μg/g and 146.56 to 981.04 μg/g, respectively, with zinc (Zn) content from 482.84 to 1 786.73 μg/g and 584.91 to 2 123.09 μg/g, and the cadmium (Cd) content from 1.77 to 4.18 μg/g and 1.87 to 4.70 μg/g, respectively. Significant differences in the accumulation of Cu, Zn, and Cd were observed between the common and low-copper groups in both sea areas (P<0.05), with differences in Cu content between the two groups ranging from 1.2 to 2.3 times, and Zn content differences ranging from 1.2 to 1.7 times. However, there were no significant differences in growth traits, minerals except for Cu, Zn, and Cd, glycogen, lipids, total protein, or ash contents between the two groups (P>0.05), indicating that the low-copper strains had both significant reduce in Cu accumulation capacity and significant changes in Zn and Cd accumulation. There were no significant differences in growth and quality traits compared to the common cultured populations. The findings contribute to deeper understanding of the mechanisms of metal accumulation in shellfish and provide essential data for the genetic improvement of copper accumulation traits in the oyster and the application of new breeding lines.
|
|
Received: 13 May 2024
|
|
|
|
|
|
[1] PENG D, ZHANG S, ZHANG H, et al. The oyster fishery in China:trend, concerns and solutions[J]. Marine Policy,2021,129:104524.
[2] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2024中国渔业统计年鉴[M].北京:中国农业出版社,2024:23-28.
[3] 林丹,孙敏秋,张克烽,等.福建牡蛎产业发展形势分析[J].中国水产,2019(3):53-57.
[4] 宋忠魁.广西茅尾海2种养殖牡蛎重金属含量评价[J].安徽农业科学,2011,39(1):317-319.
[5] PIGEOT J, MIRAMAND P, GUYOT T, et al. Cadmium pathways in an exploited intertidal ecosystem with chronic cadmium inputs (Marennes-Oléron, Atlantic coast, France)[J]. Marine Ecology Progress Series,2006,307:101-114.
[6] KUNENE S C, LIN K S, MDLOVU N V, et al. Bioaccumulation of trace metals and speciation of copper and zinc in Pacific oysters (Crassostrea gigas) using XANES/EXAFS spectroscopies [J]. Chemosphere,2021,265:129067.
[7] LUO Y, PEZACKI A T, MATIER C D, et al. A novel route of intercellular copper transport and detoxification in oyster hemocytes [J]. Journal of Hazardous Materials,2024,476:135003.
[8] LI Y, ZHANG X, MENG J, et al. Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq [J]. Science of the Total Environment,2020,701:134648.
[9] WENG N, WANG W X. Seasonal fluctuations of metal bioaccumulation and reproductive health of local oyster populations in a large contaminated estuary[J]. Environmental Pollution,2019,250:175-185.
[10] WANG W, YANG Y, GUO X, et al. Copper and zinc contamination in oysters:subcellular distribution and detoxification[J]. Environmental Toxicology and Chemistry,2011,30(8):1767-1774.
[11] LIU G, LAO Q, SU Q, et al. Spatial and seasonal characteristics of dissolved heavy metals in the aquaculture areas of Beibu Gulf, South China[J]. Human and Ecological Risk Assessment,2020,26 (7):1957-1969.
[12] WENG N, WANG W X. Variations of trace metals in two estuarine environments with contrasting pollution histories[J]. Science of the Total Environment, 2014,485:604-614.
[13] 中国营养学会.中国居民膳食营养素参考摄入量[M].北京:人民卫生出版社,2023 :280-281.
[14] CAO C, WANG W. Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR-based metabolomics[J]. Environmental Toxicology and Chemistry,2017,36(9):2428-2435.
[15] 贺美. 海洋鱼贝类痕量元素的生物可给性研究[D]. 厦门:厦门大学,2011.
[16] PAN K,WANG W X.Biodynamics to explain the difference of copper body concentrations in five marine bivalve species[J]. Environmental Science & Technology,2009,43(6):2137-2143.
[17] SHI B, HUANG Z, XIANG X, et al. Transcriptome analysis of the key role of GAT2 gene in the hyper-accumulation of copper in the oyster Crassostrea angulata[J]. Scientific Reports,2015,5:17751.
[18] 史博. 福建牡蛎(Crassostrea angulata)铜富集相关基因的研究[D]. 福建:厦门大学,2015.
[19] 吴怡迪.福建牡蛎铜和锌富集能力的遗传参数评估及其与糖原含量关系初探[D].厦门:厦门大学,2017.
[20] ABDEL-MOHSEN H A, ISMAIL M M, MOUSSA MOUSSA R. Hazardous impacts of heavy metal pollution on biometric and biochemical composition of pearl oyster Pinctada radiata from five sites along Alexandria coast, with reference to its potential health risk assessment [J]. Environmental Science and Pollution Research International,2024,31(15):23262-23282.
[21] YANG S, LI Y, CHEN F, et al. Understanding the variable metal concentrations in estuarine oysters Crassostrea hongkongensis:a biokinetic analysis[J]. Marine Environmental Research,2024,196:106393.
[22] WU Y, SHI B, ZHOU L, et al. Heritability estimates for copper/zinc accumulation capabilities and correlation with growth/quality traits in the Fujian oyster, Crassostrea angulate [J]. Aquaculture,2019,499:212-219.
[23] SUN T, JI C, LI F, et al. Bioaccumulation and human health implications of trace metals in oysters from coastal areas of China[J]. Marine Environmental Research,2023,184:105872.
[24] 王庆志.长牡蛎品种选育与生长性状的遗传参数估计[D].青岛:中国海洋大学,2012.
[25] 国家卫生和计划生育委员会.食品安全国家标准 食品中灰分的测定:GB 5009.4—2016[S].北京:中国标准出版社,2017:1-3.
[26] 国家食品药品监督管理总局.食品安全国家标准 食品中蛋白质的测定:GB 5009.5—2016[S].北京:中国标准出版社,2017:1-4.
[27] 国家卫生和计划生育委员会.食品安全国家标准 食品中脂肪的测定:GB 5009.6—2016[S].北京:中国标准出版社,2017:1-2.
[28] 阴文娅,曾果,张彩云.小鼠肝糖原测定影响因素分析[J].现代预防医学,2004,31(3):359-360.
[29] JEONG H, RA K, WON J H. A nationwide survey of trace metals and Zn isotopic signatures in mussels (Mytilus edulis) and oysters (Crassostrea gigas) from the coast of South Korea[J]. Marine Pollution Bulletin,2021,173:113061.
[30] WANG Z, AKIMOTO T, YUE T, et al. Testing combined effects of environmental trace metals/arsenic and marine trophic status on the bioaccumulation in Pacific oysters: insights from 22-site field samplings [J]. Marine Pollution Bulletin,2024,207:116827.
[31] LIU F, WANG W X. Facilitated bioaccumulation of cadmium and copper in the oyster Crassostrea hongkongensis solely exposed to zinc[J]. Environmental Science & Technology,2013,47(3):1670-1677.
[32] 蔡艳,周亦君,吴晓艺,等.3种海洋贝类重金属污染及食用风险评价研究[J].核农学报,2016,30(6):1126-1134.
[33] 杜瑞雪,范仲学,魏爱丽,等.山东沿岸经济贝类体内重金属含量分析[J].山东农业科学,2009,41(8):58-63.
[34] 翁焕新.重金属在牡蛎(Crassostrea virginica)中的生物积累及其影响因素的研究[J].环境科学学报,1996,16(1):51-58.
[35] BROWN J R. Multivariate analyses of the role of environmental factors in seasonal and site-related growth variation in the Pacific oyster Crassostrea gigas[J]. Marine Ecology Progress Series,1988,45:225-236.
[36] 翁南燕. 河口重金属污染对牡蛎生态毒理效应的研究[D]. 厦门:厦门大学,2014.
[37] COCHET M, BROWN M, KUBE P, et al.Understanding the impact of growing conditions on oysters:a study of their sensory and biochemical characteristics[J]. Aquaculture Research,2015,46(3):637-646.
[38] QIN Y, LI X, LI J, et al. Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle[J]. Food Chemistry,2021,356:129736.
[39] REN J S, MARSDEN I D, ROSS A H, et al. Seasonal variation in the reproductive activity and biochemical composition of the Pacific oyster (Crassostrea gigas) from the Marlborough Sounds, New Zealand[J]. New Zealand Journal of Marine and Freshwater Research,2003,37(1):171-182.
[40] 张智翠.太平洋牡蛎品质的季节性变化及贮藏过程中的生化变化[D].青岛:中国海洋大学,2007.
[41] LIU S, XU H, JIAN S, et al. Molecular basis of taste and micronutrient content in Kumamoto oysters (Crassostrea sikamea) and Portuguese oysters (Crassostrea angulata) from Xiangshan Bay [J]. Frontiers in Physiology,2021,12:713736.
[42] 刘雁飞. 大连湾牡蛎热风干制过程中脂质的变化与控制[D]. 大连:大连工业大学,2020.
[43] DRIDI S, ROMDHANE M S, ELCAFSI M. Seasonal variation in weight and biochemical composition of the Pacific oyster, Crassostrea gigas in relation to the gametogenic cycle and environmental conditions of the Bizert lagoon, Tunisia[J]. Aquaculture,2007,263(1/2/3/4):238-248. |
|
|
|
