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| Molecular Identification and Agglutination Characteristics of C-Type Lectin Receptor in Pacific White Shrimp Litopenaeus vannamei |
| ZHANG Huan, WANG Yaohua, YAN Maocang, HUANG Xianke, LUO Kui, LI Fang, ZHANG Min |
| Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bioresource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China |
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Abstract In order to probe into the biological function of C-type lectin, the C-type lectin LvCTLP1 gene in hepatopancreas of Pacific white shrimp Litopenaeus vannamei with body weight of 20.328—22.478 g and body length of 18.2—20.6 cm was obtained by PCR, and the full-length sequence of the genetic gene was analyzed and its sequence characteristics were analyzed, and the tissue distribution of LvCTLP1 gene and the bacterial agglutination characteristics of the recombinant protein were analyzed by fluorescence quantitative real-time PCR. Bioinformatics analysis showed that the LvCTLP1 gene encoded a 723 bp cDNA sequence, containing a 459 bp ORF that translates into a 152-amino acid polypeptide. LvCTLP1 features an N-terminal signal peptide (21 amino acids) and a C-terminal carbohydrate recognition domain (124 amino acids). The LvCTLP1 gene was shown to be the maximal expressed level in the hepatopancreas, with Ca2+-dependent bacterial agglutination activity in the recombinant rLvCTLP1 exhibited, specifically against Escherichia coli and Vibrio anguillarum. The agglutination activity of rLvCTLP1 against E. coli was found to be significantly inhibited by multiple saccharides, including D-glucose, D-galactose, D-mannose, D-fructose, maltose, sucrose, and alginose, indicating specific carbohydrate -binding capability of this lectin. These findings suggest that LvCTLP1 may function as a pattern recognition receptor capable of identifying bacterial pathogens through carbohydrate-PAMP interactions, thereby participating in antibacterial immune response of Pacific white shrimp.
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Received: 02 September 2024
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