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| Isolation and Identification of a Bacillus cereus Strain Causing Red Body Symptoms in Kuruma Shrimp Marsupenaeus japonicus |
| ZHANG Yang1, YU Jiahan1, ZHOU Xinyu1, ZHANG Huimin1, QI Junjie1, YU Fei2, ZHANG Jianxin3, GAO Huan1,4,5, XU Jing1 |
1. Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China;
2. Lianyungang Marine and Fishery Development Promotion Center, Lianyungang 222005, China;
3. Jiangsu Zhongli Marine Technology Limited Company, Lianyungang 222000, China;
4. Jiangsu Marine Biological Industry Technology Collaborative Innovation Center, Lianyungang 222005, China;
5. Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014, China |
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Abstract The suspected pathogenic bacteria were isolated, and purified from gills, hepatopancreas, intestines, and muscles of kuruma shrimp Marsupenaeus japonicus with red-body symptoms in a breeding base in Ganyu District, Lianyungang City, and identified by Gram staining, physiological and biochemical index analysis, 16S rRNA sequencing identification, re-infection experiments, and drug sensitivity tests. The results showed that the strain was identified as a Gram-positive bacterium through Gram staining, arranged singly or in long chains. Physiological and biochemical identification indicated that the bacterium decomposed glucose, starch, gelatin, methyl red, acetoin, and catalase, and had lysozyme resistance, did not decompose mannitol and Simmons′ citrate, and grew in a "villous-like" form in the motility medium. 16S rRNA sequencing showed that the strain had 99% homology with strain Bacillus cereus. Combining the morphological characteristics and physiological and biochemical indices, the strain was identified as B. cereus and named LN-20240120. The re-infection experiment showed that the kuruma shrimp and Pacific white shrimp re-infected had red-body symptoms, and a single dominant bacterium was isolated from the hepatopancreas and intestines, while this bacterium was not isolated from the control group. Bacterial hemolysis experiments and detection of the virulence gene hblC were carried out, and the detection results showed that the dominant bacterium was hemolytic and carried the virulence gene hblC, indicating that B. cereus can also infect L. vannamei. The drug sensitivity results revealed that the bacterium was sensitive to cefradine, amikacin, gentamicin, erythromycin, midecamycin, enrofloxacin, ciprofloxacin, thiamphenicol, and florfenicol, and resistant to penicillin, oxacillin, ampicillin, carbenicillin, cefuroxime, ceftazidime, polymyxin B, and compound sulfamethoxazole. The finding for the first time found that the infection of B. cereus also made the body color of shrimps red, providing a new case for the pathogen diagnosis and prevention and control of shrimp red-body syndrome.
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Received: 24 January 2025
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