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| Effects of Salinity and pH on Expression of HSP 70 Gene in Muscle, Gill and Mantle of Clam Meretrix meretrix |
| YANG Jieqing,SHEN Xinqiang,JIANG Mei,LI Lei,XU Xiafang,DONG Ran |
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Abstract As the main environmental factors affecting survival and distribution of shellfish ,huge changes in salinity and pH bring massive death to clam Meretrix meretrix .The housekeeping geneβ‐actin was used as control gene to study the suitable range of salinity and pH in pond clam farming .The transcription changes in HSP70 gene was quantified in muscle ,gill and mantle under different salinity (16 ,18 ,20 ,22 , and 24) and pH (6 .7 ,7 .7 ,8 .7 ,9 .7 ,and 10 .7) by means of real‐time fluorescence quantitative RT‐PCR method .The results proved that the HSP70 gene was induced significantly in muscle ,gill and mantle of the clam by salinity and pH .The expression of HSP70 gene was decreased significantly beyond a certain range of salinity and pH ,but it still significantly higher than that in control group .The findings showed that there were significantly higher expression of HSP70 gene in salinity of 16 ,18 ,22 ,and 24 groups than in muscle in the control group of 20(P<0 .05) .Except in gill in the salinity group of 18 and in mantle in the group of 24 ,there were no significant difference in the expression of HSP 70 gene with the blank group of 20(P>0 .05) ,the salinity group of 16 ,18 ,22 and 24 in gill and mantle there were significantly higher the expression of HSP70 gene than that in the blank group of 20 ( P< 0 .05) . However ,the expression of HSP70 gene in the in muscle ,gill and mantle in the pH group of 6 .7 ,7 .7 .9 .7 and 10 .7 showed a signifi‐cant difference with that in control group of 8 .7(P<0 .05) .
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Received: 25 April 2016
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