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| Genetic Diversity Analysis of Jellyfish Rhopilema esculentum in Liaodong Bay Based on the COⅠ Sequence |
| LI Yulong,WANG Bin,WANG Wenbo,DONG Jing |
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Abstract The 624 bp fragments of mitochondrial cytochrome oxidase Ⅰ(COⅠ) gene were sequenced and analyzed in 20 individuals jellyfish Rhopilema esculentum collected from Liaodong Bay to investigate the genetic variation and diversity .In addition ,the homologous sequences were analyzed in other 15 individu‐als sampled from the Bohai and Yellow Seas and Sea of Japan .The average contents were found to be 26 .7% in A ,36 .4% in T ,18 .8% in C and 18 .1% in G for COⅠ sequence .A total of 21 polymorphic nu‐cleotide sites were detected ,which defined 17 haplotypes .Compared with several other giant jellyfish spe‐cies ,the variation level of the jellyfish was moderate to high with H of 0 .91 ± 0 .03 and π of 0 .0056 ± 0 .0032 .With the aid of the homologous sequences retrieved from GenBank ,both phylogenetic and molec‐ular variance analysis supported significant differentiation among jellyfish populations in Sea of Japan and the Bohai Sea and Yellow Sea populations .
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Received: 25 April 2016
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