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| Sequence Analysis of Cytochrome C Oxidase Subunit Ⅰ (COⅠ) of Mitochondrial DNA Gene of Bigeye Mandarinfish Siniperca kneri in Karst Mountain |
| GUO Jiankang,AN Miao,ZHOU Qichun,CAO Hengyuan,LI Shan,CHEN Xueweijie |
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Abstract The partial mitochondrial CO Ⅰ sequences of 169 individuals of bigeye mandarinfish Siniperca kneri from three populations in Karst Mountain were amplified by PCR technique to analyze their sequence variation and the 1512 bp nucleotide sequences were obtained .The amplification showed that the average contents of A ,T ,G and C were 24 .9% ,28 .6% ,28 .7% and 17 .8% ,respectively ,the average content of AT(53 .5% ) being higher than that of GC (46 .5% ) .Five variable sites were detected and five different haplotypes were defined in 169 samples of the populations ,and the 10 variable loci were detected between the populations of S .kneri in Karst Mountain and S .kneri in Dongting lake ,most of which were occurred from 1268 nt to 1456 nt .There were no difference about codon usage among the three populations in Karst Mountain .Comparing to S .kneri in Dongting lake ,there were no significant differences in the first posi‐tions of codon usage ,higher content of T (40 .9% ) than the latter (39 .9% ) in the second codon positions , whereas the content of A and C was lower than the latter in the third codon positions .The codon usage was small discriminatory between bigeye mandarinfish in Karst Mountain and mandarinfish S .chuatsi in Dongting lake .Comparison of S .scherzeri ,S .obscura ,S .roulei and S .undulata revealed different dis‐criminatory at the first codon positions and third codon positions ,especially significantly lower content of A(30 .0% ) in S .obscura and S .undulata at the codon first positions than that (31 .8% ) in S .akneri in Karst mountain ,whereas G content (8 .9% ) of S . undulata was significantly higher than the latter (7 .3% ) .The total haplotype diversity (Hd) was 0 .395 and nucleotide diversity(Pi) was 0 .00029 ,there‐fore ,indicating a low genetic diversity level in the populations of S .kneri in Karst Mountain .
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Received: 25 June 2016
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