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| Effect of Dietary Vitamin C on Growth and Immunity of Juvenile Loach |
| WU Di,GUAN Tingting,ZHANG Siyu,ZHAO Xingwen |
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Abstract The basal diet were supplemented with 0 ,50 ,120 ,240 ,360 ,500 mg/kg vitamin C(L‐ascorbyl‐2‐polyphosphate) ,and fed loach (Misgurnus anguillicaudatus) with initial body weight of (1 .996 ± 0 .176) g twice (8:00 and 20 :00) a day for ten weeks in 18 grass jars (85 cm × 55 cm × 50 cm) containing 30 fish to investigate the effect of dietary vitamin C on growth performance (survival ,specific growth rate) ,immunity (total antioxidant capacity and activities of lysozyme and superoxide dismutase )and the tissue concentration in juvenile loach .The results showed that with the extension to the vitamin C accumulation ,the specific grow th rate (SGR) of juvenile loach increased obviously from 0 mg/kg to 132 .2 mg/kg ,significantly better than the other groups(P<0 .05) .With the increase in the vitamin C content ,the concentration in hepato‐pancreas and muscle was first increased ,and then fell down with the maximum value of 132 .2 mg/kg and 248 .8 mg/kg .So did the lysozyme ,with the maximum level of 362 .6 mg/kg(P<0 .05) ,and increase in to‐tal antioxidant capacity .We used the specific grow th rate and lysozyme activity to draw the Broken Line E‐quation to describe the best suitable supplement of vitamin C ,at the optimal dose of 95 mg/kg and 423 .3 mg/kg .
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Received: 25 June 2016
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