高邮湖大银鱼、太湖新银鱼Cytb和COⅠ基因序列多态性分析

doi:10.16378/j.cnki.1003-1111.2020.02.014

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Abstract The genetic diversity and genetic structure were analyzed in two populations of icefish Protosalanx hyalocranius and Neosalanx taihuensis in Lake Gaoyou by sequencing the Cytb and COⅠ genes in the mitochondrial DNA in order to understand the current situation of wild resources of the wild populations of icefish in the Lake Gaoyou. The full Cytb gene sequences (1141 bp) were obtained from 40 individuals of P.hyalocranius,14 variable sites were detected and 12 haplotypes were defined, with haplotype diversity of 0.871±0.031, and the nucleotide diversity of 0.00172±0.00019, showing a type of high haplotype diversity and low nucleotide diversity. The partial COⅠ gene sequences (630 bp) were obtained, 5 variable sites were detected and 6 haplotypes were defined in 40 individuals of P.hyalocranius, with haplotype diversity of 0.747±0.041, and the nucleotide diversity of 0.00202±0.00019, showing a type of high haplotype diversity and low nucleotide diversity. The full Cytb gene sequences (1141 bp) were obtained,13 variable sites were detected and 9 haplotypes were defined from 40 individuals of N. taihuensis, with haplotype diversity of 0.609±0.078, and nucleotide diversity of 0.00094±0.00027, which had a type of high haplotype diversity and low nucleotide diversity. The partial COⅠ gene sequences (630 bp) were obtained, 2 variable sites were detected and 3 haplotypes were defined in 40 individuals of N. taihuensis, with haplotype diversity of 0.232±0.085, and nucleotide diversity of 0.00038±0.00014, indicating that it had a type of low haplotype diversity and low nucleotide diversity. The neutrality test revealed that icefish P. hyalocranius and N. taihuensis in Lake Gaoyou had negative Tajima′s D and Fu′s F_s values, with significant difference, and mismatch distribution analysis map showed a peak type, suggesting P. hyalocranius and N. taihuensis have experienced population expansion in the history. The findings indicate that it should take much more measures to protect the icefish resources in Lake Gaoyou.

Key words： Protosalanx hyalocranius Neosalanx taihuensis Cytb gene COⅠ gene genetic diversity Lake Gaoyou

Received: 26 March 2019

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	Articles by authors
	LI Daming
	SUN Wenxiang
	XU Fei
	TANG Shengkai
	LIU Yanshan
	GU Xiankun
	LIU Xiaowei
	ZHANG Tongqing

Cite this article:

LI Daming,SUN Wenxiang,XU Fei, et al. Genetic Polymorphism Analysis of Cytb and COⅠ Gene Sequences of Icefish Protosalanx hyalocranius and Neosalanx taihuensis in Lake Gaoyou[J]. Fisheries Science, 2020, 39(2): 258-264.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.02.014 OR https://www.shchkx.com/EN/Y2020/V39/I2/258

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