凡纳滨对虾养殖池塘硝化细菌的分离鉴定及脱氮效果研究

doi:10.16378/j.cnki.1003-1111.2020.02.015

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Abstract Five strains of bacteria were isolated and purified from water and sediment in Pacific white leg shrimp Litopenaeus vannamei culture ponds using dilution-plate method and streak plate method, after Gram staining, and identified as Pseudomonas mendocin, Methylobacterium aminovorans, Rhizobium radiobacter, P. stutzeri and Microbacterium oxydans by morphological observation, physiological and biochemical characteristics and 16S rDNA sequence analysis to investigate the component of bacteria of water and sediment in the Pacific white leg shrimp culture pond. The water from the shrimp ponds was filtered into 250 mL conical flasks and inoculated with the five strains (with final density of 2 ×10⁷ cfu/mL) and cultured at 30 ℃ and 120 r/min for 48 hours to study their nitrification function and ammonia nitrogen removal rates.The ammonia nitrogen removal efficiency was shown to be all above 85%, 88.83% in P. mendocin and 91.73% in P. stutzeri; the removal efficiency of nitrite nitrogen were all above 90%, 95.08% in P. mendocin and 97.39% in P. stutzeri; and the removal efficiency of nitrate was above 90%, 90.49% in P. mendocin and 91.22% in P. stutzeri. The findings indicated that Pseudomonas has higher removal efficiency of ammonia nitrogen and nitrite nitrogen, and has potential application in treatment of shrimp effluent.

Key words： nitrifying bacterium isolation and purification Gram stain 16S rDNA nitrogen removal

Received: 26 June 2018

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	ZHANG Dajuan
	ZHANG Shulin
	DAI Wei
	KONG Xin
	WANG Zebin
	BI Xiangdong

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ZHANG Dajuan,ZHANG Shulin,DAI Wei, et al. Isolation, Identification and Nitrogen Removal Effect of Nitrifying Bacteria in Sediment and Water of Pacific White Shrimp Litopenaeus vannamei Culture Ponds[J]. Fisheries Science, 2020, 39(2): 265-270.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.02.015 OR https://www.shchkx.com/EN/Y2020/V39/I2/265

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