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| Genetic Diversity Analysis in Four Different Geographical Populations of Yellow Catfish Pelteobagrus vachelli by Microsatellite Markers |
| ZHENG Xiang, XU Jiejie, ZHANG Jiajia, WANG Tao, YIN Shaowu |
| College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China |
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Abstract Microsatellite markers were used to analyze genetic diversity of four populations (Jialing River, Yuanjiang River, Hanshui River and Ganjiang River) of yellow catfish Pelteobagrus vachelli. In the experiment, 80 microsatellite markers locis were randomly selected by PCR on 127 individuals from 4 populations, and 48 loci were successfully amplified with 25 screened polymorphism loci. The measurement of genetic diversity showed that the number of alleles was ranged from 2 to 18 in the four populations, the average number of effective alleles from 2.383 to 3.578, and the average observed heterozygosity ranging from 0.460 to 0.581; the average expected heterozygosity from 0.487 to 0.674; the average polymorphic information content from 0.431 to 0.627. The loci of high polymorphism were 9 in Jialing River, in Yuanjiang River, 23 in Hanshui River and 21 in Ganjiang River populations, the loci of moderate polymorphism 12, 11, 3 and 5. By calculating the genetic similarity coefficient and genetic distance between four populations and constructing the evolutionary tree, it was found that Ganjiang River population was clustered into one population, the populations of Jialing River, Yuanjiang River and Hanshui River were clustered into another population and the Yuanjiang River and the Hanshui River population were the closest. The Bayesian cluster analysis of four populations of yellow catfish was verified by Structure 2.3.4 software, consistent with the results of phylogenetic tree analysis. In this study, microsatellite markers were used to genetically evaluate four populations of yellow catfish, which provided molecular basis with the conservation, monitoring and genetic breeding of yellow catfish in the Yangtze River Basin.
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Received: 26 August 2019
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