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| Effect of Water Temperature on Embryonic Development of Frog Pelophylax nigromaculatus |
| WANG Yuzhu1,2, LIU Wenshu1, LI Siming1, LI Debing2, GUO Xiaoze1 |
1. Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
2. College of Animal Science, Sichuan Agricultural University, Chengdu 611130, China |
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Abstract The same patch eggs of frog Pelophylax nigromaculatus with the developmental 15th stage were incubated in wooden frame cotton nets disposed in a 50 cm×30 cm×44 cm glass jar about 1 cm below the water surface at a density of 100 eggs per jar at water temperatures of 18, 21, 24, 27, 30, and 33 ℃, and photoperiod of 12L∶12D to investigate the effects of different water temperatures on the hatching rate, survival rate, developmental duration, and developmental parameters including body weight, body length, tail length, and body width of the frog embryos. The results showed that the frog larvae with stage 25 had increasing survival rate first and then decreasing survival rate with the increase in water temperature, with survival rate of over 82.42% at 24—27 ℃, without significant effect of water temperature on hatching rate (P>0.05). The body weight in stage 25 was shown to be increased with the increase in the test water temperature,and body length, body width and tail length showed a trend of increasing first and then decreasing with the increase in temperature, with the maximum at 30 ℃. The embryo development rate was gradually accelerated with the increase in water temperature, and took 10 h and 60 h to develop from stage 15 to stages 19 and 25 at water temperature of 33 ℃, respectively, and 48 h and 204 h at 18 ℃. It is concluded that 24—27 ℃ is suitable water temperature range for embryo development of frog P. nigromaculatus.
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Received: 18 September 2019
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