极北海带幼苗对氮、磷和铁营养元素需求的研究

doi:10.16378/j.cnki.1003-1111.19226

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Abstract Kelp Laminaria hyperborea seedlings with length of 35 cm were cultivated in filtered and sterilized seawater in aeration at nitrogen and phosphorus concentrations [ρ(N)∶ρ(P)=10∶1] of 0 mg/L, 0.5 mg/L, 2 mg/L, 4 mg/L, 6 mg/L, 8 mg/L and 10 mg/L in NO₃^--N, with NaNO₃ and KH₂PO₄ as nitrogen and phosphorus sources at water temperature of 9 ℃, and photoperiod of 12L∶12D. After 12 h, 24 h, 36 h, 48 h and 60 h, the chlorophyll fluorescence parameters including photosystem Ⅱ maximum fluorescence yield, and F_v/F_m and the apparent photosynthetic rates of seedlings were determined in 12 h, 24 h and 48 h cultivation. In another trial, the kelp seedlings were cultivated at Fe³⁺ concentration of 0 mg/L and 1 mg/L in NH₄⁺-N (3 mg/L) groups and 0 mg/L, 0.2 mg/L, 1 mg/L and 5 mg/L NO^3--N (3 mg/L) groups, with NaNO₃ and NH₄Cl as the nitrogen source, KH₂PO₄ as phosphorus source, and FeCl₃ as iron source. Then concentrations of PO₄^3--P, NO₃-N, NH₄⁺ -N and Fe³⁺ were analyzed in the seedling culture medium in 0 h, 4 h, 10 h, 24 h and 48 h and the relative growth rate, maximum fluorescence yield (F_v/F_m) of photosystem and Fe³⁺ concentration were measured in the kelp seedlings exposed to various concentrations of nitrogen, phosphorus, and Fe³⁺ in 3 days to explore the optimal nutrient concentrations and to understand the interaction mechanism of the demand of Fe, N and P, the relative growth rate, the maximum quantum yield of (F_v/F_m) and apparent photosynthetic rate of the kelp seedlings under different nutrient concentrations. The results showed that there was significantly higher relative growth rate in the kelp seedlings exposed to 2—4 mg/L of NO₃^--N, and 0.2—0.4 mg/L of PO₄^3--P, indicating that the concentration ranges of NO₃^--N and PO₄^3--P was favorable for the seedling growth. The higher F_v/F_m and apparent photosynthetic rate were observed in the seedlings exposed to NO₃^--N concentration of 2—4 mg/L, and PO₄^3--P concentration of 0.2—0.4 mg/L, indicating that the concentration range of NO₃^--N and PO₄^3--P was favorable for photosynthesis. The good growth was found in the seedlings exposed to Fe³⁺ concentration of 0.2—1.0 mg/L, with the maximal relative growth rate at Fe³⁺ concentration of 1 mg/L. The appropriate iron concentration led to significant improvement of uptake of NO₃^--N and PO₄^3--P by the seedling within 10 h, without significant effect in 24 h. At the same time, the absorption of iron by seedlings was basically saturated within 10 h, and the absorption of NO₃^--N and PO₄³-P was inhibited at iron concentration of 5 mg/L, without significant effect of addition of iron on absorption of NH₄⁺-N (P>0.05).

Key words： Laminaria hyperborea nitrogen phosphorus iron growth physiological

Received: 21 October 2019

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	Articles by authors
	DU Xinxin
	LIU Fuli
	YUAN Yanmin
	LIANG Zhourui
	ZHANG Pengyan
	LIU Shengping

Cite this article:

DU Xinxin,LIU Fuli,YUAN Yanmin, et al. Nutrient Requirement of Kelp Laminaria hyperborea Seedlings for Nitrogen, Phosphorus and Iron[J]. Fisheries Science, 2021, 40(1): 81-88.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19226 OR https://www.shchkx.com/EN/Y2021/V40/I1/81

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