聚丙烯短期暴露对杜氏盐藻生长及抗氧化系统的影响

doi:10.16378/j.cnki.1003-1111.24061

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Abstract To explore the toxic effect of microplastic particle pollution on marine microalgae, green alga Dunaliella salinas at early exponential growth stage were exposed to polypropylene at concentration of 0 mg/L (control group), 25 mg/L, 50 mg/L and 100 mg/L at (26±1) ℃, photoperiod of 12 h L∶12 h D and light intensity of 6000 lx in an incubator for 6 days. The growth, photosynthetic pigments, chlorophyll fluorescence parameters, and antioxidant activity in D. salinas were determined in a laboratory to reveal the ecophysiological effects of polypropylene on D. salinas. The results showed that polypropylene stress had a significant impact on the growth, photosynthetic pigment content, chlorophyll fluorescence parameters, and antioxidant-related indicators in D. salina (P<0.05), with significantly reduces in 6 days of polypropylene exposure (P<0.05), especially the cells in 100 mg/L group decreased in density significatly compared to the control group. There was an enhanced inhibitory effect on the growth of D. salina as the concentration of polypropylene increased, with decrease in the relative growth rate of algal cells significantly under the concentration of 50 mg/L and 100 mg/L polypropylene exposure (P<0.05). The content of chlorophyll a, chlorophyll b, carotenoids, and total photosynthetic pigments were shown to be decreased significantly in the green alga exposed to polypropylene (P<0.05), with significantly decrease in the total photosynthetic pigments in the 50 mg/L group and 100 mg/L group compared to the control group. The upregulation of superoxide dismutase(SOD), catalase(CAT) and total antioxidant capacity(T-AOC) activities, as well as the accumulation content of malondialdehyde (MDA) demonstrated that polypropylene posed oxidative damage to D.salina. These findings show that polypropylene can affect the growth and photosynthesis of D. salina. Facilitating enzymes related to antioxidant systems, such as superoxide dismutase and catalase, enhanced the tolerance of D. salina to polypropylene.

Key words： Dunaliella salinas polypropylene growth photosynthesis antioxidant system

Received: 27 April 2024

PACS:

S946.3

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	Articles by authors
	JIANG Qingqing
	GAO Jinwei
	LIU Hongtao
	JIANG Zhifei
	ZHANG Wenhui
	ZHANG Rui
	WANG Yuqing
	ZHANG Dan
	JIA Xuying
	DOU Yong
	SHAO Peng
	XIA Sudong
	QIU Qi
	ZHOU Wenli

Cite this article:

JIANG Qingqing,GAO Jinwei,LIU Hongtao, et al. Effects of Short-Term Exposure to Polypropylene on Growth and Antioxidant System of Green Alga Dunaliella salinas[J]. Fisheries Science, 2025, 44(3): 476-483.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24061 OR https://www.shchkx.com/EN/Y2025/V44/I3/476

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