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| Opsonin-Like Molecule from Coelomic Fluid of Sea Urchin Strongylocentrotus nudus |
| LOU Yue, CHEN Dan, CHANG Yaqing, CONG Yuting, WANG Lianshun, LI Dantong |
| Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China |
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Abstract In order to study the immune mechanism of coelomic fluid of sea urchin Strongylocentrotus nudus, we researched the phagocytosis activity of phagocytes in different media and different treatments of yeast cell. The composition isolated from the non-cell coelomic fluid, regulating yeast cells and non-regulating yeast cells was analyzed with SDS-PAGE. We got the isolated opsonin-like molecule from the non-cell coelomic fluid of the sea urchin after dialysed. We determined the phagocytosis in isotonic buffer affected by opsonin-like molecule of different concentration and different time. Results showed that the phagocytosis rate of non-regulating yeast cells in isotonic buffer, cell-free coelomic fluid by yeast cell adsorption, non-cell coelomic fluid and regulating yeast cells in isotonic buffer was 1.00∶1.17∶2.00∶1.71. Only the composition isolated from the regulating yeast cells after SDS-PAGE had a distinct band in three different compositions. Added 0.5 mL, 1.0 mL, and 1.5 mL purified opsonin-like molecule in isotonic buffer, other groups were higher than the control group in each test time, and the greater the opsonin-like concentration, the higher the phagocytosis. These findings indicate that there is a kind of new opsonin-like molecule in coelomic fluid of sea urchin S. nudus with molecular weight of 156 ku which can promote phagocytosis.
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Received: 22 April 2020
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