大黄鱼致病嗜水气单胞菌的分离鉴定及其多克隆抗体制备

doi:10.16378/j.cnki.1003-1111.20156

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Abstract A dominant bacterial strain NDLc-P was isolated from the liver of diseased large yellow croaker Larimichthys crocea in Ningde, Fujian Province. An artificial infection with the isolates caused the similar symptom in the fish, with 50% lethal concentration of 3.98×10⁷ cfu/mL within 7 d trial. Physiological and biochemical tests by commercially produced kit systems (bioMerieux) revealed that strain NDLc-P was identified as Aeromonas hydrophila. Then, the 16S rDNA sequence analysis showed that the bacterium had a 99.87% identify with A. hydrophila. The phylogenetic analysis also showed that the NDLc-P isolate was clustered most clearly with A. hydrophila. Furthermore, healthy mice were immunized with formalin inactivated strain NDLc-P, and the ELISA showed that the titer of antisera was determined to be 40 000. These findings provided a reference for research on pathogenic characteristics, diagnosis and prevention and treatment of large yellow croaker disease caused by A. hydrophila.

Key words： Larimichthys crocea Aeromonas hydrophila isolation and identification polyclonal antibody

Received: 22 July 2020

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	Articles by authors
	HE Liangyin
	ZHOU Fengfang
	SHI Xiaoli
	HUANG Weiqing
	RUAN Junfeng
	TIAN Wenzhen
	LI Jinshou

Cite this article:

HE Liangyin,ZHOU Fengfang,SHI Xiaoli, et al. Isolation, Identification and Polyclonal Antibody Preparation of Aeromonas hydrophila in Large Yellow Croaker Larimichthys crocea[J]. Fisheries Science, 2022, 41(1): 69-75.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20156 OR https://www.shchkx.com/EN/Y2022/V41/I1/69

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