大菱鲆源恶臭假单胞菌的分离鉴定及全基因组分析

doi:10.16378/j.cnki.1003-1111.24010

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Abstract This study aims to determine and characterize the pathogen of the diseased turbot Scophthalmus maximus in a farm in Yantai, Shandong Province. The suspicious pathogenic bacteria were isolated from the diseased turbot and identified combined with morphological characteristics, physiological and biochemical tests, and 16S rRNA sequence. The artificial infection tests, drug sensitivity tests, and whole-genome sequencing were then carried out for the isolates. The results showed the strains of gram-negative brevibacterium was isolated from the liver, kidney, and spleen tissues of 5 diseased turbot with symptoms of abdominal distension and inflamed anus, which was motile, negative for VP reaction and methyl red reaction, and positive for nitrate reduction reaction and oxidase reaction. It was identified as Pseudomonas putida through sequencing comparison and named as D2. The strains had median lethal concentration(LD₅₀) of 8.13×10⁷ cfu/mL. D2 were sensitive to twelve antibiotics (such as quinolones, and aminoglycosides) and resistant to seven antibiotics (such as penicillin, and ampicillin). The full-length genome of strain D2 is 6.32 Mb, with G+C content accounting for 62.0%, and a total of 5878 genes were predicted. In previous studies, 947 proteins related to cell adhesion, iron absorption, and secretion functions had been identified. Previous studies had shown that the D2 strain carried resistance to various antibiotics, including quinolones and polymyxins. Based on the preliminary work, this project plans to further analyze the pathogenic mechanism of Pseudomonas putida on farmed turbot, predict its pathogenic factors and resistance genes, and provide scientific basis for the rational use of doxycycline hydrochloride in aquaculture. This study has important practical significance for the prevention and control of this disease.

Key words： Scophthalmus maximus Pseudomonas putida isolation and identification whole genome

Received: 17 January 2024

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S917.1

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	Articles by authors
	ZHAO Xiaowei
	XU Hanzhi
	HUANG Hua
	ZHANG Jianbai
	HE Jiabei
	ZHANG Lan
	ZHUANG Yanwen
	WANG He

Cite this article:

ZHAO Xiaowei,XU Hanzhi,HUANG Hua, et al. Isolation, Identification and Whole Genome Analysis of Pseudomonas putida Strain Isolated from Diseased Turbot Scophthalmus maximus[J]. Fisheries Science, 2025, 44(3): 465-475.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24010 OR https://www.shchkx.com/EN/Y2025/V44/I3/465

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