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| Effects of Ammonia Nitrogen Concentration and Substrate Selection on Ammonia Nitrogen Purification by Nitrifying Bacteria |
| MU Xianxin, MIAO Dexia, SUN Danni, ZHANG Wenping, YAN Mi, CHEN Huijuan, ZHU Ming |
| Jiangsu Key Labortaory of Marine Biotechnology, College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China |
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Abstract The attachments made of six materials of equal area including ceramsite, polyvinyl chloride, fiber, volcanic rock, non-woven fabric or fluidized bed with test nitrifying bacterium—Pseudomonas oleovorans X14-1-1 was put in a 1 L aerated bottle, mixed and cultured on a shaker at 36 °C and 130 r/min for 48 h to screen the optimal attachment of X14-1-1 by elution counting the number of bacteria attached. In the simulated ammonia nitrogen removal test, the concentration of ammonia nitrogen (NH4+-N) was determined in the 1 L aerated bottle incubators with initial ammonia nitrogen concentration of 0 (no ammonium sulfate), 10, 20, 30, 40, 50 and 60 mg/L, with 3 replicates, under the conditions of 20 °C, oxygenation for 1 L/h and light intensity of 5000 lx (photoperod 12L∶12D) on day 0, 1, 2, 3, and 4 to evaluate the nitrification efficiency. It was found that fibers and non-woven fabrics were the optimal attachment of nitrifying bacteria, and polyvinyl chloride was the most suitable attachment for ammonia nitrogen removal. In polyvinyl chloride material, the removal rate of ammonia nitrogen was shown to be 87%, 86%, 86%, 82%, 88%, 75% at the concentration of ammonia nitrogen of 10 mg/L, 20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L and 60 mg/L, respectively. The ammonia nitrogen was effectively and rapidly degraded at the initial ammonia nitrogen (NH4+-N) concentration of 50 mg/L. The strain P. oleovorans X14-1-1 had important application value in water quality regulation and water treatment in mariculture environment.
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Received: 04 June 2020
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