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| Exploration of Molecular Mechanism of Skin Albinism in Albino Northern Snakehead Channa argus var. (Teleostei: Channidae) Based on Transcriptome Sequencing |
| LI Yan1, ZHOU Yan1, LEI Luo2, SU Jian3, FAN Wei3, LUO Yu3, LI Junting1, GAO He1, ZHOU Chaowei1 |
1. College of Fisheries, Southwest University, Chongqing 402460, China;
2. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
3. Neijiang City Academy of Agricultural Sciences, Neijiang 641000, China |
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Abstract In order to understand the molecular regulatory mechanism in albino northern snakehead Channa argus, Illumina Hiseq technology was used to define the transcriptome of albino northern snakehead C. argus var., a color variety of northern snakehead skin. DEseq analysis revealed that 694 genes were found to be significantly differentially expressed. Gene ontology analysis indicated that many of these genes encoded proteins involved in cellular process, metabolic process, cell junction and transporter activity. Based on KEGG pathway analysis, DEGs were significantly enriched in tyrosine metabolism pathway, which was involved in skin albinism. The SNP and INDEL analysis showed that there was the more homozygous SNP and INDEL sites in albino northern snakehead than that in northern snakehead, but fewer number of heterozygous SNP and INDEL sites. Furthermore, the predicted genes in the tyrosine metabolic pathway, in which there were SNP non-synonymous coding in hgd and adh5 genes, but no SNPS and INDEL mutation sites were found in tyrp1 gene. Therefore, these results suggest that the skin albinism of northern snakehead be related to tyrosine metabolic pathway, and the decrease in melanin-related genes expression which reduces the skin pigments deposition. The finding will also provide a foundation for further understanding of the molecular mechanism of albino northern snakehead.
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Received: 25 January 2022
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