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| Diet Composition and Feeding Habits of Female Chinese Mitten Crab Eriocheir sinensis in the Yangtze River Estuary Based on High-Throughput Sequencing |
| XU Jingjing1,2, FENG Guangpeng1,2, ZHANG Tao1, GENG Zhi1, YANG Gang1, SONG Chao1, LI Chunbo2 |
1. Shanghai Engineering Research Center of Fisheries Resources Enhancement and Ecological Restoration of the Yangtze River Estuary,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China |
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Abstract In order to explore the feeding habits of female Chinese mitten crab Eriocheir sinensis in the Yangtze River before and after spawning, female Chinese mitten crab was collected during reproductive migration in the east beach of the Yangtze River Estuary by single boat single capsule truss bottom trawl, and the food species composition and feeding characteristics were studied before and after spawning by high-throughput sequencing technology. The results showed that the high-quality sequences of 37 samples were varied between 53 288 and 90 598. Through cluster analysis of 423 OTU, the type of live food including algae, higher plants, Piscium, Crustacea, Granulifusus kiranus, rotifer, protozoa were found after screening valid sequences. The main foods were higher plants, Crustacean and Piscium of female crab before spawning, and the main foods were higher plants and algae for female crab after spawning, the abundance index of Chao1 and ACE was significantly higher before spawning than after spawning. The results revealed that the female Chinese mitten crab had a wider feeding width and belonged to opportunistic feeders, with certain differences in the feeding habits of the female Chinese mitten crab before and after spawning. The finding provides a scientific basis for habitat restoration and resource proliferation and protection of Chinese mitten crab in the Yangtze River Estuary. In addition, high-throughput sequencing analysis methods need to be combined with traditional stomach content analysis methods to more accurately and comprehensively analyze food characteristics.
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Received: 06 August 2020
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