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| Molecular Cloning and Expression Analyses of Foxo3a and Foxo3b Genes in Gobiocypris rarus |
| QIAN Jiaming1, MIN Qianwen2, DONG Ranran1, ZENG Sheng2, ZHANG Xianbo2 |
1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Guizhou Fisheries Research Institute,Guizhou Academy of Agriculture Sciences, Guiyang 550025, China |
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Abstract The full-length cDNA of forkhead protein O3a (Foxo3a) and forkhead protein O3b (Foxo3b) genes were cloned from the ovary of Gobiocypris rarus by using RACE. RT-PCR, qRT-PCR and in situ hybridization were employed to detect the expression of the pair of paralogous genes. A 2562 bp full-length cDNA of Foxo3a gene was abtained, containing a 1953 bp open reading frame (ORF) that encoded a protein of 650 amino acids, 242 bp 5' untranslated terminal region (UTR), 367 bp 3' UTR; a 2804 bp full-length cDNA of Foxo3b gene, containing a 1959 bp ORF that encoded a protein of 652 amino acids, 470 bp 5' UTR, 375 bp 3' UTR. Foxo3a gene was only expressed in brains and ovaries with a high level among all detected tissues. Foxo3b gene was highly expressed in brains and ovaries, and also found to be expressed in all other detected tissues. Foxo3a gene mRNA was detected in the ovaries with high levels from 2 month onward and slightly increased from 4 to 5 months. There was no significance in Foxo3b gene expression level in ovaries at all developing stages. In situ hybridization results showed that both Foxo3a and Foxo3b genes were expressed in the germ cell and follicular cell of Ⅱ, Ⅲ, and Ⅳ stage oocytes. Foxo3a gene expression was affected, but Foxo3b gene not, after the treatment by rapamycin. These findings indicate that Foxo3a and Foxo3b genes may play some roles in the oogenesis of G. rarus.
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Received: 28 May 2021
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[1]叶妙荣,傅天佑.鱼丹亚科鱼类一新属新种记述(鲤形目:鲤科)[J].动物分类学报,1983,8(4):434-437.
[2]王剑伟.稀有鮈鲫的饲养管理、形态特征及遗传多样性[D].北京:中国科学院研究生院,1998:13-14.
[3]CASTRILLON D H, MIAO L L, KOLLIPARA R, et al. Suppression of ovarian follicle activation in mice by the transcription factor Foxo3a[J].Science,2003,301(5630):215-218.
[4]GEMS D, MCELWEE J J. Broad spectrum detoxification:the major longevity assurance process regulated by insulin/IGF-1 signaling?[J].Mechanisms of Ageing and Development,2005,126(3):381-387.
[5]REDDY P, ZHENG W J, LIU K. Mechanisms maintaining the dormancy and survival of mammalian primordial follicles[J].Trends in Endocrinology & Metabolism,2010,21(2):96-103.
[6]CUI C, WANG P, CUI N S, et al. Sulfated polysaccharide isolated from the sea cucumber Stichopus japonicas promotes the SDF-1α/CXCR4 axis-induced NSC migration via the PI3K/Akt/FOXO3a, ERK/MAPK, and NF-κB signaling pathways[J].Neuroscience Letters,2016,616:57-64.
[7]SHRESTHA A, NEPAL S, KIM M J, et al. Critical role of AMPK/FoxO3A axis in globular adiponectin-induced cell cycle arrest and apoptosis in cancer cells[J].Journal of Cellular Physiology,2016,231(2):357-369.
[8]BRENKMAN A B, BURGERING B M T. FoxO3a eggs on fertility and aging[J].Trends in Molecular Medicine,2003,9(11):464-467.
[9]LIU L, RAJAREDDY S, REDDY P, et al. Infertility caused by retardation of follicular development in mice with oocyte-specific expression of Foxo3a[J].Development (Cambridge, England),2007,134(1):199-209.
[10]PELOSI E, OMARI S, MICHEL M, et al. Constitutively active Foxo3 in oocytes preserves ovarian reserve in mice[J].Nature Communications,2013,4:1843.
[11]LIU Q Y, ZHANG Y, SHI B Y, et al. Foxo3b but not Foxo3a activates cyp19a1a in Epinephelus coioides[J].Journal of Molecular Endocrinology,2016,56(4):337-349.
[12]LEE S C, HSIAO C D, WANG Y S, et al. Transcriptome response of previtellogenic ovary in Anguilla japonica after artificial hormone injection[J].Marine Genomics,2017,35:31-34.
[13]钱佳铭,张显波,董然然,等.雷帕霉素对稀有鮈鲫卵子发生的影响[J].贵州农业科学,2021,49(1):88-92.
[14]TAMURA K, STECHER G, PETERSON D, et al. MEGA6:molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution,2013,30(12):2725-2729.
[15]RICHARDS J S, SHARMA S C, FALENDER A E, et al. Expression of FKHR, FKHRL1, and AFX genes in the rodent ovary:evidence for regulation by IGF-I, estrogen, and the gonadotropins[J].Molecular Endocrinology,2002,16(3):580-599.
[16]LIU H, LUO L L, QIAN Y S, et al. FOXO3a is involved in the apoptosis of naked oocytes and oocytes of primordial follicles from neonatal rat ovaries[J].Biochemical and Biophysical Research Communications,2009,381(4):722-727.
[17]MATSUDA F, INOUE N, MAEDA A, et al. Expression and function of apoptosis initiator FOXO3 in granulosa cells during follicular atresia in pig ovaries[J].The Journal of Reproduction and Development,2011,57(1):151-158.
[18]GAO L, YUAN Z H, ZHOU T, et al. FOXO genes in channel catfish and their response after bacterial infection[J].Developmental & Comparative Immunology,2019,97:38-44.
[19]PENG K, LI Y, LONG L, et al. Knockdown of FoxO3a induces increased neuronal apoptosis during embryonic development in zebrafish[J].Neuroscience Letters,2010,484(2):98-103.
[20]XIE X W, LIU J X, HU B, et al. Zebrafish foxo3b negatively regulates canonical Wnt signaling to affect early embryogenesis[J].PLoS One,2011,6(9):e24469.
[21]LI D Y, QU Y, MAO M, et al. Involvement of the PTEN-AKT-FOXO3a pathway in neuronal apoptosis in developing rat brain after hypoxia-ischemia[J].Journal of Cerebral Blood Flow and Metabolism,2009,29(12):1903-1913.
[22]FLUTEAU A, INCE P G, MINETT T, et al. The nuclear retention of transcription factor FOXO3a correlates with a DNA damage response and increased glutamine synthetase expression by astrocytes suggesting a neuroprotective role in the ageing brain[J].Neuroscience Letters,2015,609:11-17.
[23]熊振,罗仲文,蒋明贵,等.重要转录因子FoxO3a在斑马鱼性腺中的表达分析[J].生命科学研究,2012,16(1):6-8.
[24]PENG M T, SUN M M, SONG W H, et al. Molecular characterization and expression analysis of foxo3l in response to exogenous hormones in black rockfish (Sebastes schlegelii)[J].Gene,2020,753:144777.
[25]黄林艳.中国大鲵FoxO基因亚族cDNA克隆、表达及生物信息学分析[D].雅安:四川农业大学,2018.
[26]YU J, YABA A, KASIMAN C, et al. mTOR controls ovarian follicle growth by regulating granulosa cell proliferation[J].PLoS One,2011,6(7):e21415.
[27]ZHANG J, LIU W W, SUN X H, et al. Inhibition of mTOR signaling pathway delays follicle formation in mice[J].Journal of Cellular Physiology,2017,232(3):585-595.
[28]ZHANG J B, ZHUANG P W, WANG Y, et al. Reversal of muscle atrophy by Zhimu-Huangbai herb-pair via Akt/mTOR/FoxO3 signal pathway in streptozotocin-induced diabetic mice[J].PLoS One,2014,9(6):e100918.
[29]ZHANG X, CAMPRECIÓS G, RIMMELÉ P, et al. FOXO3-mTOR metabolic cooperation in the regulation of erythroid cell maturation and homeostasis[J].American Journal of Hematology,2014,89(10):954-963.
[30]FEEHAN R P, SHANTZ L M. Negative regulation of the FOXO3a transcription factor by mTORC2 induces a pro-survival response following exposure to ultraviolet-B irradiation[J].Cellular Signalling,2016,28(8):798-809. |
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