水杨酸对鼠尾藻抗高温逆性的影响

doi:10.16378/j.cnki.1003-1111.22067

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Abstract In order to investigate the response of brown alga Sargassum thunbergii to high temperature stress and the effect of salicylic acid on its resistance to high temperature stress, four wild alga strains were placed in a 1000 mL conical flask, and 0, 1, 5, and 50 mg/L salicylic acid (SA) were added at 30 ℃ (treatment group), respectively. The growth rate, photosynthetic pigment content, chlorophyll fluorescence parameters of the alga strain were determined and the antioxidant system and HSP70 gene expression was detected after being cultured at high temperature for 3 days at 15 ℃ (control group) and 30 ℃ at different concentrations.The results showed that SA treatment significantly promoted the growth of the brown alga under high temperature. There were increase first and then decrease in specific growth rate, and chlorophyll a, chlorophyll c and carotenoid contents with elevated SA concentration, with the the most significant growth promotion in 5 mg/L SA group, with 589% increase in specific growth rate compared to the high temperature treatment, increase in the chlorophyll fluorescence parameter F_v/F_m, rebounded in non-photochemical quenching (NPQ) significantly, and increase in the activities of superoxide dismutase(SOD) and catalase (CAT). The expression level of HSP70 gene was found to be significantly increased under high temperature, 1 mg/L SA further promoting the expression of the HSP70 gene, whereas 5 mg/L and 50 mg/L SA leading to reduce HSP70 gene expression. The findings provide theoretical reference with the study of large-scale artificial breeding and mechanisms of stress resistance in S. thunbergii.

Key words： Sargassum thunbergii salicylic acid high temperature stress chlorophyll fluorescence antioxidant system gene expression

Received: 27 July 2022

S968.429

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	WANG Xiaowei
	LYU Fang
	ZHOU Gefei
	WU Haiyi

Cite this article:

WANG Xiaowei,LYU Fang,ZHOU Gefei, et al. Effects of Salicylic Acid on High Temperature Resistance of Economic Brown Alga Sargassum thunbergii[J]. Fisheries Science, 2024, 43(5): 801-807.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22067 OR https://www.shchkx.com/EN/Y2024/V43/I5/801

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