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| Genetic Diversity Analysis for Wild and Cultured Filefish Thamnaconus modestus Populations Revealed by Microsatellite Markers |
| WANG Jiulong, YE Miao, LI Hongli, XU Wengang, LIU Liming, DU Rongbin, YIN Shuo |
| College of Ocean, Yantai University, Yantai 264005, China |
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Abstract Utilizing filefish Thamnaconus modestus complete genome sequence published in NCBI database, we screened microsatellite loci in the genome and designed primer pairs. Among 50 microsatellite loci selected randomly, 20 novel loci amplified target products efficiently and steadily and the 20 microsatellite markers were evaluated using a wild filefish population. The number of allele (Na) and effective allele (Ne) for these markers was found to be ranged from 4 to 13 and from 3.758 to 12.000 per locus, the observed heterozygosity (Ho) and expected heterozygosity (He) values were ranged from 0.267 to 0.833 and from 0.413 to 0.932, respectively, polymorphism information content (PIC) ranged from 0.361 to 0.910, and the Shannon-Weiner′s diversity index ranged from 0.806 to 2.520. Comparison of genetic diversity was conducted between the wild and cultured populations, the average Na and Ne were 8.2 and 7.3; the average Ne were 7.168 and 6.239; the average Ho and He were 0.663 and 0.561; the average PIC were 0.780 and 0.684; the average value of Shannon-Weiner′s diversity index were 1.914 and 1.647. The high-genetic diversity was shown to be maintained in the cultured population while polymorphism of the cultured population was lower than the wild, indicating that artificial breeding had a certain impact on the genetic diversity of the cultured population. The microsatellite markers developed in the present research could be used in population genetics and molecular marker-assisted selection for the filefish, and the findings provided theoretical basis of resources conservation and genetic breeding for T. modestus.
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Received: 23 August 2021
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