孵化湿度对胚胎期中华鳖裙边外部形态和胶原沉积的影响

doi:10.16378/j.cnki.1003-1111.22100

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Abstract The same batch of fertilized eggs [(3.05±0.05) g] laid by 5-year-old Chinese soft-shelled turtle Pelodiscus sinensis were incubated without contact in a laboratory naked incubator, incubated at 65% and 85% relative humidity, starting from the 18th stage, 20 embryos of 18, 20, 22, 24 and 26 stages were taken respectively, the calipash tissue was quickly dissected, and the collagen content was analyzed and determined by fluorescence quantitative PCR, and some samples were fixed in paraformaldehyde. The effects of two hatching humidity on the external morphology and accumulation of collagen matter in embryonic Chinese soft-shelled turtles were studied by histological analysis by Sirius red staining. It was found that the calipash related traits including embryo length, dorsal carapace index (dorsal carapace weight/body weight), calipash index (calipash weight/body weight), dorsal carapace ratio (dorsal carapace length/body length) and calipash ratio (body length/calipash width) showed an overall increasing tendency as embryos developed. The embryonic development at relative humidity of 85% had significant bigger embryo size and higher collagen content at most development stages compared with those embryos incubated at relative humidity of 65%. The results of picrosirius red staining showed that the calipash tissue of embryos incubated under relative humidity of 85% showed strong orange yellow or bright red polarized light, and the proportion of type I collagen in the calipash tissue was increased with embryo development, which was significantly higher than that in relative humidity of 65% (P<0.01). The observed rapid promoted mRNA level of type I collagen gene of Chinese soft-shelled turtle incubated at relative humidity of 85% in developmental stage 22 indicated that the biosynthesis of collagen in Chinese soft-shelled turtle embryos was induced. The findings indicated that relative incubation humidity of 85% was conducive to more collagen deposition and more preferable calipash traits during the embryonic development, which provided the possible manipulation of calipash trait development by incubation humidity changing.

Key words： Pelodiscus sinensis calipash incubation humidity collagen

Received: 17 October 2022

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	FU Kailin
	CHEN Peiheng
	YANG Mingkang
	WU Juntao
	LI Caiyan

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FU Kailin,CHEN Peiheng,YANG Mingkang, et al. Effect of Incubation Humidity on Development of Calipash Related Traits and Collagen Deposition in Embryos of Chinese Soft-Shelled Turtle Pelodiscus sinensis[J]. Fisheries Science, 2024, 43(3): 445-452.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22100 OR https://www.shchkx.com/EN/Y2024/V43/I3/445

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