急性温度胁迫对乌苏里拟鲿稚鱼抗氧化和非特异免疫功能的影响

doi:10.16378/j.cnki.1003-1111.23044

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Abstract In order to explore the effects of sharp change in water temperature on antioxidant and non-specific immune functions of juvenile bullhead fish Pseudobagrus ussuriensis, bullhead juveniles with body weight of (2.3±0.3) g were rapidly exposed to 18 ℃, 26 ℃ (control group) and 33 ℃ water stress for 24 h from 26 ℃ water in a fibergrass breeding bucket with radius of 1 m and height of 1 m, and then subjected to recovery at 26 ℃ water for 48 h. The activities of superoxide dismutase (SOD), catalase(CAT), lysozyme(LZM), alanine aminotransferase (ATF), and aspartate aminotransferase(ASF), and contents of glutathione(GSH), malondialdehyde (MAD)and total protein were detected in the stress period of 0, 3, 6, 12, and 24 h and recovery period of 3, 6, 12, and 24 h in the whole fish samples. The results showed that the activities of SOD and CAT were continuously increased during the stress period in the experimental groups, significantly higher than those in the control group (P<0.05). There was significantly higher content of malondialdehyde in the experimental groups than that in the control group at 6 and 24 h (P<0.05), while GSH content was inhibited, significantly lower than in the control group (P<0.05). At the end of the recovery period, SOD and GSH activities and MAD contents were not found to be significantly different in all experimental groups from those in control group (P>0.05), with significantly higher CAT activity in 33 ℃ group than those in control group (P<0.05). During the stress period, the activities of ATF and ASF were shown to be increased first and then decreased in all experimental groups, with significantly higher ATF activity in 18 ℃ and 33 ℃ groups than those in control group (P<0.05). The acute temperature change led to significantly decrease the LZM activity in the 18 ℃ group (P<0.05), and significantly increase the LZM activity in the 33 ℃ group (P<0.05). At the end of the recovery period, the ASF and LZM activities recovered to the control level in all experimental groups. There was significantly higher ASF activity in 33 ℃ group than that in control group (P<0.05). It was found that the acute temperature stress led to significant impact on the antioxidant enzymes and non-specific immune function of the juvenile Pseudobagrus ussuriensis, in which the high temperature stress caused irreversible damage to the immune function. It is recommended to pay more attention to changes in water temperature in daily aquaculture to avoid sharp temperature changes causing damage to the juvenile fish of P. ussuriensis.

Key words： Pseudobagrus ussuriensis temperature stress antioxidant enzyme immune function

Received: 25 May 2023

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	CHEN Junping
	WU Huihui
	SHEN Fangfang
	ZHANG Jiaxin
	YU Ruomeng
	FU Yongjie
	ZHAO Daoquan
	XIE Guoqiang
	HE Yiqing
	LI Xiujie

Cite this article:

CHEN Junping,WU Huihui,SHEN Fangfang, et al. Effects of Acute Temperature Stress on Antioxidant Enzyme Activities and Non-Specific Immune Function in Juvenile Bullhead Fish Pseudobagrus ussuriensis[J]. Fisheries Science, 2024, 43(4): 580-589.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.23044 OR https://www.shchkx.com/EN/Y2024/V43/I4/580

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