氨氮对中华绒螯蟹蜕壳和抗氧化能力的影响

doi:10.16378/j.cnki.1003-1111.22105

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Abstract To evaluate the effects of different concentrations of ammonia-N in the water environment on the molting, proximate composition, and antioxidant capacity of juvenile Chinese mitten crab Eriocheir sinensis, the Chinese mitten crab [(6.13±0.05) g] was cultured in water with ammonia-N concentration of 0 (the control group), 10.47, 20.93, 31.40, and 41.87 mg/L, respectively. And the survival rate, growth index, proximate composition, and antioxidant enzyme activities of Chinese mitten crab were determined at 0, 3, 6, 9 and 12 days after exposure to ammonia-N at water temperature of (22.0±0.5) ℃ and pH of 7.63±0.44. The results showed that the survival rate and molting rate of Chinese mitten crab in the 10.47 mg/L ammonia-N treatment group were almost unaffected during ammonia-N exposure, whereas those in the other ammonia-N treatment groups were significantly decreased (P<0.05). The consumption of energy substances in crab meat, namely crude protein and crude fat, decreased with the increase in ammonia-N concentration. The superoxide dismutase (SOD) activity in the hepatopancreas of crabs in the 10.47 mg/L ammonia-N treatment group was significantly increased at the initial stage of exposure, the antioxidant capacity of Chinese mitten crab was enhanced, and the malondialdehyde (MDA) content was not affected after exposure. However, with the increase in ammonia-N concentration, SOD, catalase (CAT), and peroxidase (POD) activities were significantly decreased (P<0.05), and MDA content was significantly increased (P<0.05). Therefore, the ammonia-N concentration of higher than 20.93 mg/L may inhibit the growth of Chinese mitten crab, reduce its antioxidant enzyme activities, and induce oxidative stress. The findings provide the data support and theoretical basis for the study on response mechanisms of Chinese mitten crab to ammonia-N stress and the improvement of the aquaculture water environment.

Key words： ammonia-N Eriocheir sinensis growth performance proximate composition antioxidant

Received: 03 November 2022

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	WANG Tianyu
	CONG Yaxin
	WU Zhaoxia
	SUN Wentao
	LIU Yimeng

Cite this article:

WANG Tianyu,CONG Yaxin,WU Zhaoxia, et al. Effects of Ammonia-N Concentrations on Molting and Antioxidant Capacity of Chinese Mitten Crab Eriocheir sinensis[J]. Fisheries Science, 2024, 43(4): 590-597.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.22105 OR https://www.shchkx.com/EN/Y2024/V43/I4/590

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