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| Effects of Transport Time on Stress Injury and Immune Function of Red Swamp Crayfish Procambarus clarkii |
| ZHU Minli1, HU Honghui2, CHEN Huangen3, SU Shengyan1,2,4, ZHU Jian2 |
1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
3. Jiangsu Fishery Technology Promotion Center, Nanjing 226007, China;
4. Panjin Raoyang Agricultural Science and Technology Development Co., Ltd., Panjin 124107, China |
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Abstract To investigate the effects of transport time on the cumulative survival rate, antioxidant capacity, and immune-related gene expression of red swamp crayfish Procambarus clarkia, red swamp crayfish with body weight of (8.26±2.67) g were placed in a shipping basket in a car for 0 (control), 2, 8, and 14 h of dry transportation at temperature of 20 °C. Survival, antioxidant metabolic enzyme activities, and immune-related gene indicators in hemolymph and hepatopancreas as well as changes in histological structure of hepatopancreas were investigated in the red swamp crayfish during 0, 2, 8, and 14 h of transportation. The cumulative survival rate was determined in the red swamp crayfish reared for 10 d after 0, 2, 8, and 14 h of transportation. The results showed that there was significantly higher cumulative survival rate in 2 hours than that after 8 hours and 14 hours, with the cumulative survival rate of only 2.22% in the red swamp crayfish exposed to 14 hour transportation in the 10th days. Histological observation showed that the hepatopancreas were severely damaged in the 8-hour group. There were significantly higher activities of total superoxide dismutase, catalase, acid phosphatase, glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase and content of malondialdehyde as well as total antioxidant capacity in the hepatopancreas in the crayfish in the 14-hour group than those in the control group (P<0.05). The expression levels of GPX, CuZnSOD, HSP70, Astacidin, and Lysozyme genes in the hepatopancreas were shown to be significantly higher in the crayfish in the 14-hour group than those in the control group (P<0.05). It was found that transportation time greater than 8 hours led to significantly high cumulative survival rate, damaged hepatopancreas, antioxidant ability, and expression of immune-related genes. Therefore, it is suggested that the transportation time in actual transportation should be lower than 8 hours. The findings may provide reference for research and development to improve the transportation survival rate of red swamp crayfish.
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Received: 20 August 2024
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